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Publication Date:
June 2005
ISSN:
1437-4331
DOI:
10.1515/CCLM.2002.181

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Clinical Chemistry and Laboratory Medicine (CCLM)

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Precision and Accuracy of a HPLC Method for Measurement of Fecal Porphyrin Concentrations

Floris M. J. Zuijderhoudt / Johannes S. Kamphuis / Willem E. Kluitenberg / Jenneke Dorresteijn-de Bok

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 40, Issue 10, Pages 1036–1039, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2002.181, June 2005

Publication History:
Published Online:
2005-06-01

Abstract

We studied precision and accuracy of a HPLC method for determination of porphyrins in feces. A commercial standard solution appeared to contain less coproporphyrin (15%) than stated by the manufacturer. The between-batch coefficients of variation were often below 15% and were higher than the within-batch coefficients. The precision of porphyrin measurements was not influenced by the type of porphyria. Recoveries of added coproporphyrin and protoporphyrin were 90% and 108%; coefficients of variation were 6% and 19%, respectively.

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