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Journal of Pediatric Endocrinology and Metabolism

Editor-in-Chief: Kiess, Wieland

Editorial Board Member: Darendeliler, Feyza / Gustafsson, Jan / Luo, Feihong / Mericq, Veronica / Lanes M. D., Roberto / Battelino, Tadej / Buyukgebiz, Atilla / Cassorla, Fernando / Chrousos, George P. / Cutfield, Wayne / Fideleff, Hugo L. / Hershkovitz, Eli / LaFranchi, Stephen H. / Mohn, Angelika / Root, Allen W. / Rosenfeld, Ron G. / Wabitsch, Martin / Werther, George / Zadik, Zvi

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Evidence of insulin-like growth factor binding protein-3 proteolysis during growth hormone stimulation testing

1 / Leslie A. Soyka1 / Amanda Angelescu1 / Mary M. Lee1

1Division of Endocrinology, Department of Pediatrics, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, USA

Corresponding author: Dr. Benjamin U. Nwosu, Department of Pediatrics, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA Phone: +1-508-3347872, Fax: +1-508-8564287

Citation Information: Journal of Pediatric Endocrinology and Metabolism. Volume 24, Issue 3-4, Pages 163–167, ISSN (Online) 2191-0251, ISSN (Print) 0334-018X, DOI: 10.1515/jpem.2011.088, April 2011

Publication History

Published Online:
2011-04-14

Abstract

Objectives: The ternary complex is composed of insulin-like growth factor (IGF)-I, IGF binding protein (IGFBP)-3 and acid labile subunit (ALS). Growth hormone (GH) promotes IGFBP-3 proteolysis to release free IGF-I, ALS, and IGFBP-3 fragments. Our aim was to determine whether elevated GH levels during GH stimulation testing would trigger IGFBP-3 proteolysis.

Design: This prospective study of 10 short prepubertal children (height standard deviation score –2.37±0.31) used arginine and GH releasing hormone stimulation to study dynamic changes in the ternary complex moieties. IGFBP-3 was measured in two assays: a radioimmunoassay (RIA) that detects both cleaved and intact IGFBP-3; and an immunochemiluminescence assay (ICMA) that detects only intact IGFBP-3.

Results: IGFBP-3 measured by RIA increased by 19% (p<0.05), while IGFBP-3 measured by ICMA did not significantly increase (6.1%).

Conclusion: The significant increase in IGFBP-3 measured by RIA, but not ICMA, provides evidence of IGFBP-3 proteolysis during acute GH stimulation.

Keywords: growth hormone; proteolysis; short stature

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