High correlations were obtained between probes in seemingly unrelated probe sets, following an examination of the data from thousands of Affymetrix GeneChips. Investigation revealed that these unexpected correlations were between probes that were adjacent to high-valued probes. Using carefully selected probes, together with simple linear models, the extent of blur has been measured for each CEL file. The cause is shown to be attributable to poorly performing scanners. Blur can result in the doubling of the values of thousands of probes. This in turn can lead to the doubling of the expression level for hundreds of probe sets.

Editor-in-Chief: Stumpf, Michael P.H.
Editorial Board Member: Beaumont, Mark / Binder, Harald / Gupta, Mayetri / Hubbard, Alan E. / Husmeier, Dirk / Ji, Hongkai / Keles, Sunduz / Kerr, Kathleen / Lazzeroni, Laura / Lin, Shili / Ma, Ping / Marjoram, Paul / Mertens, Bart / Nerman, Olle / G. Petretto, Enrico / Plagnol, Vincent / Purdom, Elizabeth / Robin, Stéphane / Rzhetsky, Andrey / Sanguinetti, Guido / van der Laan, Mark J. / von Haeseler, Arndt / Weeks, Daniel E. / Wiuf, Carsten / Zhao, Hongyu
6 Issues per year
IMPACT FACTOR 2011: 1.517
5-year IMPACT FACTOR: 1.704
Rank 27 out of 116 in category Statistics & Probability in the 2011 Thomson Reuters Journal Citation Report/Science Edition
Issues
Volume 12 (2013)
Volume 11 (2012)
Volume 10 (2011)
Volume 9 (2010)
Volume 8 (2009)
Volume 7 (2008)
Volume 6 (2007)
Volume 5 (2006)
Volume 4 (2005)
Volume 3 (2004)
Volume 2 (2003)
Volume 1 (2002)
Most Downloaded Articles
- A General Framework for Weighted Gene Co-Expression Network Analysis by Zhang, Bin and Horvath, Steve
- Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray Experiments by Smyth, Gordon K
- Detecting Differential Expression in RNA-sequence Data Using Quasi-likelihood with Shrunken Dispersion Estimates by Lund, Steven P./ Nettleton, Dan/ McCarthy, Davis J. and Smyth, Gordon K.
- Adjusting for Spurious Gene-by-Environment Interaction Using Case-Parent Triads by Shin, Ji-Hyung/ Infante-Rivard, Claire/ Graham, Jinko and McNeney, Brad
- A Shrinkage Approach to Large-Scale Covariance Matrix Estimation and Implications for Functional Genomics by Schäfer, Juliane and Strimmer, Korbinian
The Detection of Blur in Affymetrix GeneChips
1University of Essex
1University of Essex
Citation Information: Statistical Applications in Genetics and Molecular Biology. Volume 9, Issue 1, Pages –, ISSN (Online) 1544-6115, DOI: 10.2202/1544-6115.1590, October 2010
- Published Online:
- 2010-10-18
Keywords: microarray; correlation; blur; scanner


















Comments (0)