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Acta Medica Martiniana

The Journal of Comenius University in Bratislava

3 Issues per year

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Open Access

Changes in Several Inflammatory and Oxidation Markers after Ovalbumin-Sensitization in a Guinea Pig Model of Allergic Asthma - A Pilot Study

D Mokra1 / A Drgova1 / R Pullmann1 / P Mikolka1 / M Antosova1 / J Mokry1

Department of Physiology, Jessenius Faculty of Medicine, Comenius University, Martin1

Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University, Martin2

Department of Clinical Biochemistry, Jessenius Faculty of Medicine, Comenius University and University Hospital, Martin3

Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia4

This content is open access.

Citation Information: Acta Medica Martiniana. Volume 12, Issue 2, Pages 5–11, ISSN (Print) 1335-8421, DOI: 10.2478/v10201-011-0032-6, September 2012

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Published Online:

Changes in Several Inflammatory and Oxidation Markers after Ovalbumin-Sensitization in a Guinea Pig Model of Allergic Asthma - A Pilot Study

Ovalbumin (OVA)-sensitization is a common way to evoke changes similar to changes in allergic asthma in humans. Activated cells produce various pro-inflammatory and vasoactive substances including reactive oxygen species. The goal of this pilot study was to evaluate mobilization of leukocytes into the lungs and oxidation processes induced by OVA-sensitization in a guinea-pig model of allergic asthma. Guinea-pigs were divided into OVA-sensitized and naïve animals. After sacrificing animals, blood samples were taken and total and differential leukocyte counts were calculated, and eosinophil cationic protein (ECP) and total antioxidant status (TAS) in the plasma were determined. Left lungs were saline-lavaged and total number of cells and differential leukocyte count in the bronchoalveolar lavage fluid (BAL) were calculated. Right lung tissue was homogenized, ECP, TAS and products of lipid and protein oxidation (thiobarbituric acid-reactive substances and lysine-lipoperoxidation end-products) were determined in the lung homogenate. OVA-sensitization increased a total number of cells and percentages of eosinophils and neutrophils and slightly increased ECP in the blood and in the BAL fluid. In addition, increased lipid and protein oxidation in the lung homogenate, and decreased TAS in the plasma was found in OVA-sensitized compared to naïve animals. In conclusion, OVA-sensitization increased mobilization of leukocytes into the lungs and elevated production of reactive oxygen spesies (ROS), accompanied by a decrease in plasma TAS.

Keywords: allergic asthma; ovalbumin-sensitization; oxidation stress; reactive oxygen species; guinea pig

  • Holgate ST. Pathogenesis of asthma. Clin Exp Allergy 2008; 38: 872-897.

  • Rahman I, Biswas SK, Kode A. Oxidant and antioxidant balance in the airways and airway diseases. Eur J Pharmacol 2006; 533: 222-239.

  • Park CS, Kim TB, Lee KY, et al. Increased oxidative stress in the airway and development of allergic inflammation in a mouse model of asthma. Ann Allergy Asthma Immunol 2009; 103: 238-247. [Web of Science]

  • Ciencewicki J, Trivedi S, Kleeberger SR. Oxidants and the pathogenesis of lung diseases. J Allergy Clin Immunol 2008; 122: 456-468. [Web of Science]

  • Talati M, Meyrick B, Peebles RS Jr., et al. Oxidant stress modulates murine allergic airway responses. Free Radic Biol Med 2006; 40: 1210-1219.

  • Cho YS, Moon HB. The role of oxidative stress in the pathogenesis of asthma. Allergy Asthma Immunol Res 2010; 2: 183-187. [Web of Science]

  • Mokry J, Nosalova G. Evaluation of the cough reflex and airway reactivity in toluene- and ovalbumin-induced airway hyperresponsiveness. J Physiol Pharmacol 2007; 58 Suppl 5: 419-426. [PubMed]

  • Mokra D, Drgova A, Mokry J, et al. Combination of budesonide and aminophylline diminished acute lung injury in animal model of meconium aspiration syndrome. J Physiol Pharmacol 2008;59 Suppl 6: 461-471. [PubMed]

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-275.

  • Giulivi C, Davies KJ. Dityrosine: a marker for oxidatively modified proteins and selective proteolysis. Methods Enzymol 1994; 233: 363-371.

  • Dousset N, Ferretti G, Taus M, Valdiguiè P, Curatola G. Fluorescence analysis of lipoprotein peroxidation. Methods Enzymol 1994; 233: 459-469.

  • Das DK. Cellular, biochemical and molecular aspects of reperfusion injury. Ann NY Acad Sci 1994; 723: 118-124.

  • Stone KD, Prussin C, Metcalfe DD. IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol 2010; 125: S73-S80.

  • Nguyen LT, Lim S, Oates T, Chung KF. Increase in airway neutrophils after oral but not inhaled corticosteroid therapy in mild asthma. Respir Med 2005; 99: 200-207.

  • Jahnz-Rózyk K, Plusa T, Mierzejewska J. Eotaxin in serum of patients with asthma or chronic obstructive pulmonary disease: relationship with eosinophil cationic protein and lung function. Mediators Inflamm 2000; 9:175-179

  • Babusikova E, Jesenak M, Kirschnerova R, Banovcin P, Dobrota D. Association of oxidative stress and GST-T1 gene with childhood bronchial asthma. J Physiol Pharmacol 2009; 60 Suppl 5: 27-30.

  • Nadeem A, Raj HG, Chhabra SK. Increased oxidative stress in acute exacerbations of asthma. J Asthma 2005; 42: 45-50.

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