Jump to ContentJump to Main Navigation

Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society

4 Issues per year

Increased IMPACT FACTOR 2012: 1.162

Open Access
VolumeIssuePage

Open Access

Hepatoprotective effect of the ethyl acetate extract of Teucrium polium L. against carbontetrachloride-induced hepatic injury in rats

Tatjana Panovska1 / Svetlana Kulevanova1 / Icko Gjorgoski1 / Mirjana Bogdanova1 / Gordana Petrushevska1

Institute of Toxicology Faculty of Pharmacy, University »Ss. Cyril and Methodius«, 1000 Skopje, Republic of Macedonia1

Institute of Pharmacognosy Faculty of Pharmacy, University »Ss. Cyril and Methodius«, 1000 Skopje, Republic of Macedonia2

Institute of Biology Faculty of Natural Sciences and Mathematics, University »Ss. Cyril and Methodius«, P. B. 126, 1000 Skopje Republic of Macedonia3

Clinical Center, Institute of Clinical Biochemistry, 1000 Skopje Republic of Macedonia4

Institute of Pathology, University »Ss. Cyril and Methodius«, 1000 Skopje, Republic of Macedonia5

This content is open access.
(CC BY-NC-ND 4.0)

Citation Information: Acta Pharmaceutica. Volume 57, Issue 2, Pages 241–248, ISSN (Online) 1846-9558, ISSN (Print) 1330-0075, DOI: 10.2478/v10007-007-0020-x, May 2007

Publication History:
Published Online:
2007-05-16

Hepatoprotective effect of the ethyl acetate extract of Teucrium polium L. against carbontetrachloride-induced hepatic injury in rats

Hepatoprotective activity of the ethyl acetate extract of Teucrium polium L. was investigated using rats with CCl4--induced liver damage. Specific biochemical parameters (glutathione peroxidase, superoxide dismutase, reduced glutathione and total antioxidative status) were estimated in blood and in liver homogenate. Lipid peroxidation in CCl4--intoxicated rats was evidenced by a marked increment in the levels of thiobarbituric acid reactive substances. Histopatological examinations of the liver were undertaken to monitor the liver status. Silymarin was used as a standard to compare the hepatoprotective activity of the extract. Some biochemical parameters in groups treated with the Teucrium polium extract at a dose of 25 mg kg-1, showed significantly different values than that of the CCl4--treated group. The liver biopsy of all experimental rat groups treated with the Teucrium polium ethyl acetate extract showed significant restoration of the normal histomorphological pattern of liver cells. The study substantiates the potential hepatoprotective activity of the ethyl acetate extract of Teucrium polium L.

Hepatoprotective effect of the ethyl acetate extract of Teucrium polium L. against carbontetrachloride-induced hepatic injury in rats

Hepatoprotektivno djelovanje etil-acetatnog ekstrakta biljke Teucrium polium L. ispitivano je na Štakorima čija je jetra oštećena tetraklormetanom. Specifični biokemijski parametri (glutation peroksidaza, superoksid dismutaza, reducirani glutation i ukupni antioksidativni status) procijenjeni su u krvi i homogenatu jetre. Lipidna peroksidacija u Štakora intoksiciranih tetraklormetanom dokazana je povećanjem koncentracije reaktivnih derivata tiobarbiturne kiseline. Stanje jetre procijenjeno je histopatološkim ispitivanjima. U ispitivanjima hepatoprotektivnog djelovanja ekstrakta Teucrium polium upotrebljen je silimarin kao standard. Neki biokemijski parametri u skupini životinja tretiranih ispitivanim ekstraktom u dozi od 25 mg kg-1 imali su značajno različite vrijednosti od skupine tretirane s CCl4. Biopsija jetre u svim ispitivanim životinjama tretiranim ekstraktom pokazala je uspostavu normalnih histomorfoloških uvjeta u stanicama, Što ukazuje na hepatoprotektivno djelovanje biljke Teucrium polium.

Keywords: Teucrium polium (Lamiaceae); hepatoprotective effect; carbon tetrachloride; sylimarin; glutathione

Keywords: Teucrium polium Lamiaceae; hepatoprotektivni učinak; tetraklormetan; silimarin; glutation

  • T. Sunita, V. Vupta and B. Sandeep, Comparative study of antioxidant potential of tea with and without additivies, Indian. J. Physiol. Pharmacol.44 (2000) 215-219.

  • B. Halliwell and J. M. C. Gutteridge, Free Radicals in Biology and Medicine, 2nd ed., Oxford University Press, Oxford 1989, pp. 78-93.

  • S. K. Mitra, M. V. Venkataranganna, R. Sudaram and S. Gopumadhavan, Protective effect of HD-03, a herbal formulation, against various hepatotoxic agents in rats, J. Ethnopharmacol.63 (1998) 181-186; DOI: 10.1016/S0378-8741(98)00088-9

  • B. Uday, D. Das and K. R. Banerjee, Reactive oxygen species: Oxidative damage and pathogenesis, Curr. Sci.77 (1999) 658-666.

  • C. Fraga, B. Leibovitz and A. Tappel, Halogenated compounds as inducers of lipid peroxidation in tissue slices, Free Rad. Biol. Med.3 (1987) 119-123.

  • P. Hochstein and A. S. Atallah, The nature of oxidants and antioxidant systems in the inhibition of mutation and cancer, Mutation Res.202 (1988) 363-375; DOI: 10.1016/0027-5107(88)90198-4.[CrossRef]

  • M. S. Suleiman, A. S. Abdul-Ghani, S. Al-Khalil and R. Amin, Effect of Teucrium polium boiled leaf extract on intestinal motility and blood pressure, J. Ethnopharmacol.22 (1988) 111-116; DOI: 10.1016/0378-8741(88)90236-X.[CrossRef]

  • M. Tariq, A. M. Ageel and M. S. Al-Said, Antiinflammatory activity of T. polium, Int. J. Tissue React.11 (1989) 185-188.

  • M. N. Gharaibeh, H. H. Elayan and A. S. Salhab, Hypoglycemic effects of Teucrium polium, J. Ethnopharmacol.24 (1988) 93-99; DOI: 10.1016/0378-8741(88)90139-0.[CrossRef]

  • Z. Yaniv, A. Dafni, J. Friedman and D. Palevitch, Plants used for the treatment of diabetes in Isreal, J. Ethnopharmacol.19 (1987) 145-151; DOI: 10.1016/0378-8741(87)90038-9.[CrossRef]

  • G. Autore, F. Capasso, R. De Fusco, M. P. Fasulo, M. Lembo, N. Mascolo and A. Menghini, Antipyretic and antibacterial actions of Teucrium polium (L.), Pharmacol. Res. Commun.16 (1984) 21-29; DOI: 10.1016/S0031-6989(84)80101-0.[PubMed] [CrossRef]

  • G. Ilhami, U. Metin, O. Munir, B. Suktru and K. Irfan, Antioxidant and antimicrobial activities of Teucrium polium L., J. Food Technol.1 (2003) 9-16.

  • T. Kadifkova Panovska, S. Kulevanova and M. Stefova, In vitro antioxidant activity of some Teucrium species (Lamiaceae), Acta Pharm.55 (2005) 207-214.

  • T. Kadifkova Panovska and S. Kulevanova, Effect of some Teucrium species (Lamiaceae) on lipid peroxidation in rat liver microsomes, Fresenius Environ. Bull.14 (2005) 957-959.

  • R. O. Recknagel, A new direction in the study of carbon tetrachloride hepatotoxicity, Life Sci.33 (1983) 401-408; DOI: 10.1016/0024-3205(83)90787-7.[CrossRef]

  • M. J. Ellenhorn, Ellenhorn's Medical Toxicology: Diagnosis and Treatment of Human Poisoning, 2nd ed., Williams and Wilkins Publications, Los Angeles 1997.

  • E. Beutler, O. Duron and B. M. Kelly, Improved method for the determination of blood glutathione, J. Lab. Clin. Med.61 (1963) 882-888.

  • H. Ohkawa, N. Ohishi and K. Yagi, Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction, Anal. Biochem.95 (1979) 351-358; DOI: 10.1016/0003-2697(79)90738-3.[PubMed] [CrossRef]

  • M. M. Bradford, A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding, Anal. Biochem.72 (1976) 248-254; DOI: 10.1016/0003-2697(76)90527-3.[CrossRef]

  • A. Valenzuela, C. Lagos, K. Schmidt and K. Videla, Sylimarin protection against hepatic lipid peroxidation induced by acute ethanol intoxication in the rat, Biochem. Pharmacol.3 (1985) 2209-2212. DOI: 10.1016/0006-2952(85)90421-6.[CrossRef]

Comments (0)

Please log in or register to comment.