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Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society

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Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract

Sunil Kumar1 / Dinesh Kumar1 / Man Jusha1 / Kamal Saroha1 / Nidhan Singh1 / Bhoodev Vashishta1

Department of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119 Haryana, India1

Department of Botany, Kurukshetra University, Kurukshetra-136119 Haryana, India2

This content is open access.

Citation Information: Acta Pharmaceutica. Volume 58, Issue 2, Pages 215–220, ISSN (Online) 1846-9558, ISSN (Print) 1330-0075, DOI: 10.2478/v10007-008-0008-1, May 2008

Publication History

Published Online:
2008-05-30

Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract

Citrullus colocynthis (L.) Schrad. (Cucurbitaceae) is a medicinal plant traditionally used as an abortifacient and to treat constipation, oedema, bacterial infections, cancer and diabetes. Preliminary phytochemical screening of the plant showed the presence of large amounts of phenolics and flavonoids. Subsequent quantification showed the presence of 0.74% (m/m) phenolics (calculated as gallic acid) and 0.13% (m/m) flavonoids calculated as catechin equivalents per 100 g of fresh mass. The presence of phenolic compounds prompted us to evaluate its antioxidant activity. In the present study, methanolic fruit extract of C. colocynthis was screened to evaluate its freeradical scavenging effect. The highest antioxidant and free radical scavenging ability of the fruit extract was observed at a concentration of 2500 μg mL-1.

Antioksidativni potencijal i sposobnost hvatanja slobodnih radikala metanolnog ekstrakta plodova Citrullus colocynthis (L.) Schrad.

Citrullus colocynthis (L.) Schrad. (Cucurbitaceae) je ljekovita biljka koja se tradicionalno upotrebljava kao abortiv i za liječenje konstipacije, edema, bakterijskih infekcija, karcinoma i dijabetesa. Preliminarno fitokemijsko pretraživanje ukazalo je na prisutnost velikih količina fenola i flavonoida. Udio fenola bio je 0,74% (preračunato na galnu kiselinu), a flavonoida 0,13% preračunato na ekvivalente katehina na 100 g svježe mase. Zbog prisutnosti fenolnih spojeva ispitivano je antioksidativno djelovanje i sposobnost hvatanja slobodnih radikala metanolnog ekstrakta plodova. Koncentracija 2500 μg mL-1 imala je najjači učinak.

Keywords: Citrullus colocynthis fruit; Cucurbitaceae; methanolic extract; antioxidants; free radical scavenging

Keywords: plod biljke Citrullus colocynthis (Cucurbitaceae); metanolni ekstrakt; antioksidansi; hvatanje slobodnih radikala

  • B. Halliwell and J. M. C. Gutteridge, Free Radicals in Biology and Medicine, 3rd ed., Oxford University Press, Oxford 1999.

  • T. Finkel and N. J. Holbrook, Oxidants, oxidative stress and the biology of aging, Nature 408 (2000) 239-247; DOI:10.1038/35041687. [CrossRef]

  • T. P. A. Devasagayam, J. C. Tilak, K. K. Boloor, K. S. Sane, S. S. Ghaskadbi and R. D. Lele, Review: Free radicals and antioxidants in human health: Current status and future prospects, J. Assoc. Phys. India 52 (2004) 794-804.

  • A. L. Miller, Antioxidant flavonoids: structure, function and clinical usage, Alt. Med. Rev. 1 (1996) 103-111.

  • A. Delazar, S. Gibbons, A. R. Kosari, H. Nazemiyeh, M. Modarresi, L. Nahar and S. D. Sarker, Flavone C-glycosides and cucurbitacin glycosides from Citrullus colocynthis, DARU 14 (2006) 109-114.

  • V. L. Singleton and J. A. Rossi, Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents, Am. J. Enol. Vitic. 16 (1965) 144-158.

  • D. Marinova, F. Ribarova and M. Atanasova, Total phenolics and flavonoids in Bulgarian fruits and vegetables, J. Univ. Chem. Tech. Metall. 40 (2005) 255-260.

  • N. Sreejayan and M. N. A. Rao, Free radical scavenging activity of curcuminoids, Drug Res. 46 (1996) 169-171.

  • S. M. Klein, G. Cohen and A. I. Cederbaum, Production of formaldehyde during metabolism of dimethylsulphoxide by hydroxyl radical generating system, Biochemistry 20 (1981) 6006-6012. [CrossRef]

  • I. Gülçin, H. A. Alici and M. Cesur, Determination of in vitro antioxidant and radical scavenging activities of propofol, Chem. Pharm. Bull. 53 (2005) 281-285; DOI: 10.1248/cpb.53.281. [CrossRef]

  • M. Nishikimi, N. A. Rao and K. Yagi, The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen, Biochem. Biophys. Res. Commun. 46 (1972) 849-853. [PubMed]

  • R. Govindarajan, S. Rastogi, M. Vijayakumar, A. Shirwaikar, A. K. Rawat, S. Mehrotra and P. Pushpangadan, Studies on antioxidant activities of Desmodium gangeticum, Biol. Pharm. Bull. 26 (2003) 1424-1427; DOI: 10.1248/bpd.26.1424. [CrossRef] [PubMed]

  • L. W. Aurand, N. H. Boonme and G. G. Gidding, Superoxide and singlet oxygen in milk lipid peroxidation, J. Dairy Sci. 60 (1977) 363-369.

  • A. E. Hagerman, K. M. Riedl, G. A. Jones, K. N. Sovik, N. T. Ritchard, P. W. Hartzfeld and T. L. Riechel, High molecular weight plant polyphenolics (tannins) as biological antioxidants, J. Agric. Food Chem. 46 (1998) 1887-1892; DOI: 10.1021/jf970975b. [CrossRef]

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