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Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society

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Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms

Basavaraj Hiremath1 / Bennikallu Mruthyunjayaswamy1

Department of Chemistry, Gulbarga University, Gulbarga-585106, India1

This content is open access.

Citation Information: Acta Pharmaceutica. Volume 58, Issue 3, Pages 275–285, ISSN (Online) 1846-9558, ISSN (Print) 1330-0075, DOI: 10.2478/v10007-008-0017-0, December 2008

Publication History

Published Online:
2008-12-22

Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms

Two spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first method is based on the reaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with ceftazidime in the presence of ferric chloride in acidic medium. The resulting blue complex absorbs at λmax 628 nm. The second method describes the reaction between the diazotized drug and N-(1-naphthyl)ethylenediamine dihydrochloride (NEDA) to yield a purple colored product with λmax at 567 nm. The reaction conditions were optimized to obtain maximum color intensity. The absorbance was found to increase linearly with increasing the concentration of CFZM; the systems obeyed the Beer's law in the range 2-10 and 10-50 μg mL-1 for MBTH and NEDA methods, resp. LOD, LOQ and correlation coefficient values were 0.15, 0.79 and 0.50, 2.61. No interference was observed from common excipients present in pharmaceutical formulations. The proposed methods are simple, sensitive, accurate and suitable for quality control applications.

Razvoj i validacija spektrofotometrijskih metoda za određivanje ceftazidima u farmaceutskim doziranim pripravcima

Razvijene su dvije spektrofotometrijske metode za određivanje ceftazidima (CFZM), čistog ili u farmaceutskim pripravcima. Prva metoda se temelji na reakciji 3-metilbenzotiazolin-2-on hidrazona (MBTH) sa ceftazidimom u prisutnosti željezovog(III) klorida u kiselom mediju. Nastaje plavi kompleks s maksimumom apsorpcije pri λmax 628 nm. Druga metoda se temelji na reakciji između diazotiranog lijeka i N-(1-naftil)etilendiamin dihidroklorida (NEDA), pri čemu nastaje ljubičasti produkt s λmax pri 567 nm. Reakcijski uvjeti su optimirani da se dobije maksimalni intenzitet boje. Apsorbancija raste linearno s porašću koncentracije CFZM; sustavi slijede Beerov zakon u koncentracijskom području 2-10 za MBTH metodu i 1050 μg mL-1 za NEDA metodu. LOD i LOQ te vrijednosti korelacijskog koeficijenta su 0,15, 0,79 i 0,50, 2,61. Uobičajene pomoćne tvari ne smetaju određivanju ceftazidima. Predložene metode su jednostavne, osjetljive, točne i pogodne za primjenu u kontroli kvalitete.

Keywords: ceftazidime; diazotization; spectrophotometry; pharmaceutical formulation

Keywords: ceftazidim; diazotacija; spektrofotometrija; farmaceutski pripravak

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