Jump to ContentJump to Main Navigation
Show Summary Details

Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society

IMPACT FACTOR increased in 2015: 1.212
5-year IMPACT FACTOR: 1.620

SCImago Journal Rank (SJR) 2014: 0.353
Source Normalized Impact per Paper (SNIP) 2014: 0.743
Impact per Publication (IPP) 2014: 1.275

Open Access
See all formats and pricing

Select Volume and Issue
Loading journal volume and issue information...

Antioxidant properties of methanolic extracts of the leaves of seven Egyptian Cassia species

Maher El-hashash1 / Mahfouz Abdel-Gawad1 / Mortada El-Sayed1 / Waffa Sabry1 / El-Sayed Abdel-Hameed1 / Ezzat Abdel-Lateef1

Chemistry Department, Faculty of Science, Ain Shams University Egypt1

Laboratory of Medicinal Chemistry, Theodor Bilharz Research Institute, Warrak El-Hader Giza, Egypt2

This content is open access.

Citation Information: Acta Pharmaceutica. Volume 60, Issue 3, Pages 361–367, ISSN (Online) 1846-9558, ISSN (Print) 1330-0075, DOI: 10.2478/v10007-010-0030-y, December 2010

Publication History

Published Online:

Antioxidant properties of methanolic extracts of the leaves of seven Egyptian Cassia species

In the present study, antioxidant activity of methanolic extracts of the leaves of seven Egyptian Cassia species was investigated using two methods, the phosphomolyb-date method and 1,1 diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity method.· The results revealed that C. glauca is the most potent species and that the activity of other plant species decreases in the following order: C. grandis > C. nodosa > C. fistula > C. didymobtrya > C. occidentalis > C. sophera.

Defatted methanolic extract of the most active plant C. glauca was subjected to fractionation using different organic solvents such as CHCl3, EtOAc and BuOH. Antixidant activities of the fractions were investigated and the results showed that ethyl acetate fraction possessed high activity. Total phenolic and flavonoid concentrations of each plant extract were determined using the Folin-Ciocalteu reagent and aluminum chloride. Correlation between radical scavenging capacities of extracts and total phenolic concentration was observed.

Antioksidativno djelovanje metanolnih ekstrakata listova sedam egipatskih vrsta roda Cassia

U radu je ispitano antioksidativno djelovanje metanolnih ekstrakata listova sedam egipatskih vrsta roda Cassia koristeći fosfomolibdatnu metodu i metodu vezanja slobodnih 1,1-difenil-2-pikrilhidrazil radikala (DPPH). Rezultati pokazuju da C. glauca ima najveć u aktivnost te da se djelovanje smanjuje sljedećim redom: C. grandis > C. nodosa > C. fistula > C. didymobtrya > C. occidentalis > C. sophera. Odmašćeni metanolni ekstrakt najaktivnije biljke C. glauca frakcioniran je pomoću različitih organskih otapala kao što su CHCl3, EtOAc i BuOH. Ispitivanje antioksidativnog djelovanja pojedinih frakcija pokazuje da je etil-acetatna frakcija najaktivnija. Pomoću Folin-Ciocaltuovog reagensa i aluminijevog klorida određena je ukupna koncentracija fenola i flavonoida svakog pojedinog ekstrakta. Uočena je korelacija između sposobnosti hvatanja slobodnih radikala i ukupnog sadržaja fenola.

Keywords: Cassia species; antioxidant; phenolics; flavonoids; DPPH

Keywords: Cassia vrste; antioksidans; fenoli; flavonoidi; DPPH

  • S. E. Lee, H. J. Hwang, J. S. Ha, H. S. Jeog and J. H. Kim, Screening of medicinal plant extracts for antioxidant activity, Life Sci. 73 (2003) 167-179; DOI: 10.1016/S0024-3205(03)00259-5. [PubMed] [CrossRef]

  • M. B. Tadhani, V. H. Patel and R. Subhash, In vitro antioxidant activities of Stevia rebaudiana leaves, J. Food Comp. Anal. 20 (2007) 323-329; DOI: 10.1016/j.jfca.2006.08.004. [CrossRef]

  • N. G. Baydar, G. Ozkan and S. Yasar, Evaluation of the antiradical and antioxidant potential of grape extracts, Food Cont. 18 (2007) 1131-1136; DOI: 10.1016/j.foodcont.2006.06.011. [CrossRef]

  • A. Kumaran and J. Karunakaran, In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India, LWT - Food Sci. Tech. 40 (2007) 344-352; DOI: 10.1016/j.lwt.2005.09.011. [CrossRef]

  • D. Prakash, S. Suri, M. Upadhyay and B. N. Singh, Total phenol, antioxidant and free radical scavenging activities of some medicinal plants, Int. J. Food Sci. Nutr. 58 (2007) 18-28; DOI: 10.1080/09637480601093269. [Web of Science] [CrossRef]

  • C. V. Junior, A. Rezende, D. H. S. Silva and I. C. V. S. Bolzani, Ethnopharmacological, biological and chemical aspects of the Cassia genus, Quím. Nova 29 (2006) 1279-1286.

  • P. Siddhuraju, P. S. Mohan and K. Becker, Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp, Food Chem. 79 (2002) 61-67; DOI: 10.1016/S0308-8146(02)00179-6. [CrossRef]

  • J. F. Mupangwa, T. Acamonic, J. H. Topps, N. T. Ngongoni and H. Hamndikuwanda, Content of soluble and bound condensed tannins of three tropical herbaceous forage legumes, Feed Anim. Sci. Tech. 83 (2000) 139-144; DOI: 10.1016/S0377-8401(99)00117-0. [CrossRef]

  • H. Moriyama, T. Iizuka, M. Nagai and Y. Murata, HPLC quantification of kampherol -3-O-gentiobioside in Cassia alata, Fitoterapia 74 (2003) 425-430; DOI: 10.1016/S0367-326X(03)00058-3. [CrossRef]

  • D. Huang, B. Ou and R. L. Prior, The chemistry behind antioxidant capacity assays, J. Agr. Food Chem. 53 (2005) 1841-1856; DOI: 10.1021/jf030723c. [CrossRef]

  • A. Shirwaikar, K. Rajendran and I. S. Punithaa, In vitro antioxidant studies on the benzyl tetra isoquinoline alkaloid berberine, Biol. Pharm. Bull. 29 (2006) 1906-1910; DOI: 10.1248/bpb.29.1906. [PubMed] [CrossRef]

  • P. Prieto, M. Pineda and M. Aguilar, Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex. Specific application to the determination of vitamin E, Anal. Biochem. 269 (1999) 337-341; DOI: 10.1006/abio.1999.4019. [CrossRef]

  • H. O. Edeoga, D. E. Okwu and B. O. Mbaebie, Phytochemical constituents of some Nigerian medicinal plants, Afr. J. Biotech. 4 (2005) 685-688.

  • K. S. Kumar, K. Ganesan and P. V. Rao, Antioxidant potential of solvent extracts of Kappaphycus alverezii (Doty) Doty-An edible seaweed, Food Chem. 107 (2008) 289-295; DOI: 10.1016/j.foodchem.2007.08.016. [CrossRef] [Web of Science]

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Beauty E Omoruyi, Graeme Bradley, and Anthony J Afolayan
BMC Complementary and Alternative Medicine, 2012, Volume 12, Number 1, Page 215
Maria G.L. Brandão, Marc Pignal, Sergio Romaniuc, Cristiane F.F. Grael, and Christopher W. Fagg
Journal of Ethnopharmacology, 2012, Volume 143, Number 2, Page 488
Beatriz Souza, Tiago De Oliveira, Thiago Aquino, Maria de Lima, Ivan Pitta, Suely Galdino, Edeltrudes Lima, Teresinha Gonçalves-Silva, Gardênia Militão, Luciana Scotti, Marcus Scotti, and Francisco Mendonça
Acta Pharmaceutica, 2012, Volume 62, Number 2

Comments (0)

Please log in or register to comment.