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Acta Pharmaceutica

The Journal of Croatian Pharmaceutical Society


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Antioxidant properties of methanolic extracts of the leaves of seven Egyptian Cassia species

Maher El-hashash1 / Mahfouz Abdel-Gawad1 / Mortada El-Sayed1 / Waffa Sabry1 / El-Sayed Abdel-Hameed1 / Ezzat Abdel-Lateef1

Chemistry Department, Faculty of Science, Ain Shams University Egypt1

Laboratory of Medicinal Chemistry, Theodor Bilharz Research Institute, Warrak El-Hader Giza, Egypt2

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Citation Information: Acta Pharmaceutica. Volume 60, Issue 3, Pages 361–367, ISSN (Online) 1846-9558, ISSN (Print) 1330-0075, DOI: 10.2478/v10007-010-0030-y, December 2010

Publication History

Published Online:
2010-12-06

Antioxidant properties of methanolic extracts of the leaves of seven Egyptian Cassia species

In the present study, antioxidant activity of methanolic extracts of the leaves of seven Egyptian Cassia species was investigated using two methods, the phosphomolyb-date method and 1,1 diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity method.· The results revealed that C. glauca is the most potent species and that the activity of other plant species decreases in the following order: C. grandis > C. nodosa > C. fistula > C. didymobtrya > C. occidentalis > C. sophera.

Defatted methanolic extract of the most active plant C. glauca was subjected to fractionation using different organic solvents such as CHCl3, EtOAc and BuOH. Antixidant activities of the fractions were investigated and the results showed that ethyl acetate fraction possessed high activity. Total phenolic and flavonoid concentrations of each plant extract were determined using the Folin-Ciocalteu reagent and aluminum chloride. Correlation between radical scavenging capacities of extracts and total phenolic concentration was observed.

Antioksidativno djelovanje metanolnih ekstrakata listova sedam egipatskih vrsta roda Cassia

U radu je ispitano antioksidativno djelovanje metanolnih ekstrakata listova sedam egipatskih vrsta roda Cassia koristeći fosfomolibdatnu metodu i metodu vezanja slobodnih 1,1-difenil-2-pikrilhidrazil radikala (DPPH). Rezultati pokazuju da C. glauca ima najveć u aktivnost te da se djelovanje smanjuje sljedećim redom: C. grandis > C. nodosa > C. fistula > C. didymobtrya > C. occidentalis > C. sophera. Odmašćeni metanolni ekstrakt najaktivnije biljke C. glauca frakcioniran je pomoću različitih organskih otapala kao što su CHCl3, EtOAc i BuOH. Ispitivanje antioksidativnog djelovanja pojedinih frakcija pokazuje da je etil-acetatna frakcija najaktivnija. Pomoću Folin-Ciocaltuovog reagensa i aluminijevog klorida određena je ukupna koncentracija fenola i flavonoida svakog pojedinog ekstrakta. Uočena je korelacija između sposobnosti hvatanja slobodnih radikala i ukupnog sadržaja fenola.

Keywords: Cassia species; antioxidant; phenolics; flavonoids; DPPH

Keywords: Cassia vrste; antioksidans; fenoli; flavonoidi; DPPH

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