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Ethanol Withdrawal-Associated Drinking and Drinking in the Dark: Common and Discrete Genetic Contributions

1 / Pamela Metten1 / Lawrence C. Huang1 / Jason P. Schlumbohm1 / Stephanie E. Spence1 / Amanda M. Barkley-Levenson1 / Deborah A. Finn1 / Justin S. Rhodes2 / Andy J. Cameron1

1Portland Alcohol Research Center, VA Medical Center, and Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR 97239, USA

2Neuroscience Program, The Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

This content is open access.
(CC BY-NC-ND 4.0)

Citation Information: Addiction Genetics. Volume 1, Pages 3–11, ISSN (Online) 2084-7688, DOI: 10.2478/addge-2012-0002, October 2012

Publication History:
Published Online:
2012-10-19

Ethanol Withdrawal-Associated Drinking and Drinking in the Dark: Common and Discrete Genetic Contributions

Individual mice differ in the dose of ethanol they will ingest voluntarily when it is offered during limited access periods in the circadian dark, a phenotype called drinking in the dark (DID). Substantial genetic variation in DID has been reported across a few standard inbred mouse strains, and a line of High Drinking in the Dark (HDID) mice has been established through selective breeding on the blood ethanol concentration (BEC) they attain at the end of a drinking session. Here, we report ethanol DID data for 23 inbred mouse strains, including 11 not previously reported, corroborating the genetic contributions to this trait. We also report data on a different ethanol drinking trait, the increased intake seen after multiple cycles of chronic intermittent exposure to ethanol vapor (CIE). Drinking escalated significantly during ethanol withdrawal. However, HDID mice and their HS controls showed equivalent escalation during withdrawal, demonstrating that withdrawal-associated drinking escalation is not a clear genetic correlate of selection on DID. Across inbred strains, DID is substantially genetically correlated with previously-published twobottle ethanol preference drinking data assessed under conditions of continuous ethanol access. Although inbred strain data for withdrawalassociated drinking are not available, the current pattern of results suggests that withdrawal-associated drinking is genetically distinct from DID, while genetic contributions to DID and two-bottle preference drinking are substantially similar.

Keywords: Withdrawal; Drinking; Preference; Genetics; Mouse; Selective breeding; Inbred strains; Chronic intermittent exposure; Dependence

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