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Archives of Industrial Hygiene and Toxicology

The Journal of Institute for Medical Research and Occupational Health

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Bla g 1 Allergen Levels in Zagreb Area Household Dust

Ljerka Prester1 / Jelena Macan1

Institute for Medical Research and Occupational Health, Zagreb, Croatia1

This content is open access.

Citation Information: Archives of Industrial Hygiene and Toxicology. Volume 62, Issue 1, Pages 33–39, ISSN (Print) 0004-1254, DOI: 10.2478/10004-1254-62-2011-2080, March 2011

Publication History

Published Online:
2011-03-21

Bla g 1 Allergen Levels in Zagreb Area Household Dust

Cockroach allergy is a health problem in many parts of the world. In urban environments, indoor exposure to cockroach allergens involves a risk of asthma. The aim of this study was to measure the mass fraction of Bla g 1, a major allergen of the German cockroach (Blatella germanica) in 30 house samples, collected at random from Zagreb area households, Croatia. Dust samples were collected on cellulose filters by vacuuming living rooms floors. After extraction, Bla g 1 was detected using the commercial enzyme-linked immunosorbent assay (ELISA). Only four of the thirty households had detectable Bla g 1 levels, and only in one was its concentration higher than 2.0 U g-1, the threshold associated with sensitisation. The Bla g 1 ELISA proved highly sensitive, with the detection limit of 0.12 U g-1. The within- and between-assay imprecision was 8.9 % and 14.4 %, respectively, and accuracy 85 % to 120 %. Low Bla g 1 levels in the household dust support previously reported low prevalence of skin sensitisation to B. germanica among Zagreb residents. Further monitoring should reveal if there are differences in cockroach allergen exposure and sensitisation between households from other geographic areas in Croatia.

Razine alergena Bla g 1 (Blatella germanica) u kućnoj prašini s područja grada Zagreba

Alergija na žohare zdravstveni je problem u mnogim dijelovima svijeta. Prisutnost alergena žohara u kućanstvu važan je faktor rizika za nastanak astme u gradskoj sredini. Cilj ovoga rada bio je odrediti maseni udjel Bla g 1, glavnog alergena smeđeg žohara (Blatella germanica) u 30 uzoraka kućne prašine uzorkovanih u neselektivno izabranim kućanstvima sa šireg područja Zagreba. Uzorci prašine skupljeni su usisavanjem prašine s poda u dnevnom boravku na celulozne filtre. Nakon ekstrakcije, Bla g 1 je određen komercijalnim enzim-imunokemijskim testom. Maseni udjeli alergena Bla g 1 bili su mjerljivi u samo 4 od 30 kućanstava (13,3 %). Koncentracija Bla g 1 > 2 U g-1 koja se povezuje s pojavom senzibilizacije u ljudi ustanovljena je u jednom od 30 kućanstava (3,3 %). Bla g 1 ELISA vrlo je osjetljiva metoda s granicom detekcije od 0.12 U g-1, a dobiveni su zadovoljavajući rezultati za analitičku nepreciznost i netočnost.

Rezultati ove pilot-studije pokazuju da je razina alergena Bla g 1 u kućnoj prašini s područja Zagreba vrlo niska, što je sukladno ranijim podatcima o niskoj učestalosti kožne reaktivnosti na smeđeg žohara u odrasloj populaciji iz istog područja. Zbog moguće razlike u izloženosti i senzibilizaciji stanovništva alergenima žohara ovisno o geografskom području, trebalo bi provesti daljnja ispitivanja.

Keywords: allergy; cockroaches; ELISA; indoor allergens

Keywords: alergeni unutarnjih prostora; alergija; ELISA; žohari

  • Cohn RD, Arbes SJ Jr, Jaramillo R, Reid LH, Zeldin DC. National prevalence and exposure risk for cockroach allergen in U. S. Households. Environ Health Perspect 2006;11: 522-6.

  • Macan J, Varnai VM, Maloča I, Kanceljak-Macan B. Increasing trend in atopy markers prevalence in a Croatian adult population between 1985-1999. Clin Exp Allergy 2007;37:1756-63.

  • Gold DR. Environmental tobacco smoke, indoor allergens, and childhood asthma. Environ Health Perspect 2000;108(Suppl 4):643-51. [PubMed]

  • Litonjua AA, Carey VJ, Burge HA, Weiss ST, Gold DR. Exposure to cockroach allergen in the home is associated with incident doctor-diagnosed asthma and recurrent wheezing. J Allergy Clin Immunol 2001;107:41-7. [CrossRef] [PubMed]

  • Diette GB, Hansel NN, Buckley TJ, Curtin-Brosnan J, Eggleston PA, Matsui EC, McCormack MC, Williams AL, Breysse PN. Home indoor pollutant exposure among innercity children with and without asthma. Environ Health Perspect 2007;115:1665-9. [CrossRef]

  • Leung R, Ho P, Lam CWK, Lai CKW. Sensitization to inhaled allergens as a risk factor for asthma and allergic diseases in Chinese population. J Allergy Clin Immunol 1997;99: 594-9. [PubMed] [CrossRef]

  • Arruda LK, Vailes LD, Ferriani VPL, Santos ABR, Pomés A, Chapman MD. Cockroach allergens and asthma. J Allergy Clin Imminol 2001;107:419-28. [CrossRef]

  • Wu CH, Lee MF. Molecular characteristics of cockroach allergens. Cel Mol Immunol 2005;2:177-80.

  • Satinover SM, Reefer AJ, Pomes A, Chapman MD, Platts-Mills TAE, Woodfolk JA. Specific IgE and IgG antibody-binding patterns to recombinate cockroach allergens. J Allergy Clin Immunol 2005;115:803-9. [CrossRef]

  • Pollart SM, Mullins DE, Vailes LD, Hayden ML, Platts-Mills TAE, Sutherland WM, Chapman MD. Identification, quantitation, and purification of cockroach allergens using monoclonal antibodies. J Allergy Clin Immunol 1991;87: 511-21. [PubMed] [CrossRef]

  • Melén E, Pomés A, Vailes LD, Arruda LK, Chapman MD. Molecular cloning of Per a 1 and definition of the cross-reactive Group 1 cockroach allergens. J Allergy Clin Immunol 1999;103:859-64. [CrossRef]

  • De Lucca SD, Taylor DJM, O'Meara TJ, Jones AS, Tovey ER. Measurement and characterization of cockroach allergens detected during normal domestic activity. J Allergy Clin Immunol 1999;104:672-80. [PubMed] [CrossRef]

  • Li M, Gustchina A, Alexandratos J, Wlodawer A, Wünschmann S, Kepley CL, Chapman MD, Pomés A. Crystan structure of a dimerized cockroach allergen Bla g 2 complexed with a monoclonal antibody. J Biol Chem 2008;283:22806-14. [Web of Science]

  • Eggleston PA, Rosenstreich D, Lynn H, Gergen P, Baker D, Kattan M, Mortimer KM, Mitchell H, Ownby D, Slavin R, Malveaux F. Relationship of indoor allergen exposure to skin test sensitivity in inner-city children with asthma. J Allergy Clin Immunol 1998;102:563-70. [CrossRef] [PubMed]

  • Rosenstreich DL, Egglestone P, Kattan M, Baker D, Slavin RG, Gergen P, Mitchell H, McNiff-Mortimer K, Lynn H, Ownby D, Malveaux F. The role of cockroach allergy and exposure to cockroach allergen in causing morbidity among inner-city children with asthma. N Engl J Med 1997;336:1356-63. [CrossRef] [PubMed]

  • Matsui EC, Wood RA, Rand C, Kanchanaraksa S, Swartz L, Curtin-Brosnan J, Eggleston PA. Cockroach allergen exposure and sensitization in suburban middle-class children with asthma. J Allergy Clin Immunol 2003;112:87-92. [CrossRef] [PubMed]

  • Sporik R, Squillace SP, Ingram JM, Rakes G, Honsinger RW, Platts-Mills TAE. Mite, cat, and cockroach exposure, allergen sensitization, and asthma in children: a case-control study of three schools. Thorax 1999;54:675-80. [CrossRef]

  • Leaderer BP, Belanger K, Triche E, Holford T, Gold DR, Kim Y, Jankun T, Ren P, McSharry J, Platts-Mills TAE, Chapman MD, Bracken MB. Dust mite, cockroach, cat, and dog allergen concentrations in homes of asthmatic children in the northeastern United States: impact of socioeconomic factors and population density. Environ Health Perspect 2002;110:419-25. [PubMed] [CrossRef]

  • Gruchalla RS, Pongracic J, Plaut M, Evans R, Visness CM, Walter M, Crain EF, Kattan M, Morgan WJ, Steinbach S, Stout J, Malindzak G, Smartt E, Mitchell H. Inner city asthma study: Relationships among sensitivity, allergen exposure, and asthma morbidity. J Allergy Clin Immunol 2005;115: 478-85. [CrossRef] [PubMed] [Web of Science]

  • Peters JL, Levy JI, Rogers CA, Burge HA, Spengler JD. Determinants of allergen concentrations in apartments of asthmatic children living in public housing. J Urban Health 2007;84:185-97. [PubMed] [CrossRef] [Web of Science]

  • Huss K, Adkinson FN, Eggleston PA, Dawson C, Van Natta ML, Hamilton RG. House dust mite and cockroach exposure are strong risk factors for positive allergy skin test responses in the childhood asthma management program. J Allergy Clin Immunol 2001;107:48-54. [PubMed] [CrossRef]

  • Call RS, Smith TF, Morris E, Chapman MD, Platt-Mills TAE. Risk factors for asthma in inner city children. J Pediatr 1992;121:862-6. [PubMed] [CrossRef]

  • Rogers L, Cassino C, Berger KI, Goldring RM, Norman RG, Klugh T, Reibman J. Asthma in the elderly: cockroach sensitization and severity of airway obstruction in elderly nonsmokers. Chest 2002;122:1580-86. [CrossRef] [PubMed]

  • Lan JL, Lee DT, Wu CH, Chang CP, Yeh CL. Cockroach hypersensitivity: preliminary study of allergic cockroach asthma in Taiwan. J Allergy Clin Immunol 1988;82:736-40. [PubMed] [CrossRef]

  • Tandon N, Maitra SB, Saha GK, Modak A, Hati AK. Role of cockroaches in allergy to house dust in Calcutta, India. Ann Allergy 1990;64:155-7. [PubMed]

  • Gergen PJ, Mortimer KM, Eggleston PA, Rosenstreich D, Mitchell H, Ownby D, Kattan M, Baker D, Wright EC, Slavin R, Malveaux F. Results of the National Cooperative Inner-City Asthma Study (NCICAS) environmental intervention to reduce cockroach allergen exposure in inner-city homes. J Allergy Clin Immunol 1999;103:501-6. [PubMed] [CrossRef]

  • Arbes SJ Jr, Sever M, Mehta J, Gore JC, Schal C, Vaughn B, Mitchell H, Zeldin DC. Abatement of cockroach allergens (Bla g 1 and Bla g 2) in low-income, urban housing: Month 12 continuation results. J Allergy Clin Immunol 2004;113: 109-14.

  • Pollart SM, Smith TF, Morris EC, Gelber LE, Platts-Mills TAE, Chapman MD. Environmental exposure to cockroach allergens: analysis with monoclonal antibody-based enzyme immunoassays. J Allergy Clin Immunol 1991;87:505-10. [PubMed] [CrossRef]

  • Schou C, Fernandez-Caldas E, Lockey RF, Løwenstein H. Environmental assay for cockroach allergens. J Allergy Clin Immunol 1991;87:828-34. [CrossRef] [PubMed]

  • Dreborg S. Mite allergens. Collection, determination, expression of results and risk levels for sensitization and symptom induction. Allergy 1998;53(Suppl 48):88-91. [CrossRef] [PubMed]

  • Fahlbusch B, Heinrich J, Groß I, Jäger L, Richter K, Wichmann HE. Allergens in house-dust samples in Germany: results of an East-West German comparison. Allergy 1999;54:1215-22. [PubMed] [CrossRef]

  • Stelmach I, Jerzynska J, Stelmach W, Majak P, Chew G, Gorski P, Kuna P. Cockroach allergy and exposure to cockroach allergen in Polish children with asthma. Allergy 2002;57:701-5. [CrossRef] [PubMed]

  • Kitch BT, Chew G, Burge HA, Muilenberg ML, Weiss ST, Platts-Mills TAE, O'Connor G, Gold DR. Socioeconomic predictors of high allergen levels in homes in the greater Boston area. Environ Health Perspect 2000;108:301-7. [PubMed] [CrossRef]

  • Sarpong SB, Hamilton RG, Eggleston PA, Adkinson NF. Socioeconomic status and race as risk factors for cockroach allergen exposure and sensitization in children with asthma. J Allergy Clin Immunol 1996;97:1393-401. [CrossRef] [PubMed]

  • Wang C, El-Nour MM, Bennett GW. Survey of pest infestation, asthma, and allergy in low-income housing. J Community Health 2008;33:31-9. [CrossRef] [PubMed] [Web of Science]

  • Sarpong SB, Wood RA, Karrison T, Eggleston PA. Cockroach allergen (Bla g 1) in school dust. J Allergy Clin Immunol 1997;99:486-92. [PubMed] [CrossRef]

  • Macan J, Plavec D, Kanceljak B, MIlković-Kraus S. Exposure levels and skin reactivity to german cockroach (Blatella germanica) in Croatia. Croat Med J 2003;44:756-60. [PubMed]

  • Prester Lj, Brčić Karačonji I, Macan J. Determination of mite allergens in house dust using the enzyme immunoassay. Arhiv Hig Rada Toksikol 2007;58:413-9.

  • De Blay F, Sanchez J, Hedelin G, Perez-Infante A, Vérot A, Chapman M, Pauli G. Dust and airborne exposure to allergens derived from cockroach (Blattella germanica) in low-cost public housing in Strasbourg (France). J Allergy Clin Immunol 1997;99:107-12. [PubMed] [CrossRef]

  • Custovic A, Green R, Taggart SC, Smith A, Pickering CA, Chapman MD, Woodcock A. Domestic allergens in public places. II: Dog (Can f 1) and cockroach (Bla g 2) allergens in dust and mite, cat, dog and cockroach allergens in the air in public buildings. Clin Exp Allergy 1996;26:1246-52. [PubMed] [CrossRef]

  • Lamiaa B, Mariam L, Ahmed A. Bacteriological analysis of Periplaneta americana L. (Dictyoptera; Blattidae) and Musca domestica L. (Diptera; Muscidae) in ten districts of Tangier, Morocco. Afr J Biotechol 2007;6:2038-42.

  • Pomés A, Chapman MD, Vailes LD, Blundell TL, Dhanara V. Cockroach allergen Bla g 2. Am J Respir Crit Care Med 2002;165:391-7. [PubMed] [CrossRef]

  • Prester Lj, Macan J. Determination of Alt a I (Alternaria alternata) in poultry farms and a sawmill using ELISA. Med Mycol 2010;48:298-302. [CrossRef] [Web of Science]

  • Prester Lj, Macan J, Matković K, Vučemilo M. Determination of Aspergillus fumigatus allergen 1 in poultry farms using the enzyme immunoassay. Arh Hig Rada Toksikol 2010;61: 167-73.

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