Jump to ContentJump to Main Navigation

Advances in Medical Sciences

The Journal of Medical University of Bialystok

2 Issues per year

IMPACT FACTOR 2012: 0.796
5-year IMPACT FACTOR: 1.094

Open Access

Open Access

IL-8, IL-12 and IL-10 cytokines generation by neutrophils, fibroblasts and neutrophils- fibroblasts interaction in psoriasis

E Glowacka1 / P Lewkowicz1 / H Rotsztejn1 / A Zalewska1

Department of Clinical Immunology, Polish Mother's Memorial Hospital - Research Institute, Lodz, Poland1

Department of Cosmetology, Medical University of Lodz, Lodz, Poland2

Department of Dermatology and Venerology, Medical University of Lodz, Lodz, Poland3

This content is open access.

Citation Information: Advances in Medical Sciences. Volume 55, Issue 2, Pages 254–260, ISSN (Online) 1898-4002, ISSN (Print) 1896-1126, DOI: 10.2478/v10039-010-0037-0, October 2010

Publication History

Published Online:

IL-8, IL-12 and IL-10 cytokines generation by neutrophils, fibroblasts and neutrophils- fibroblasts interaction in psoriasis

Purpose: Psoriasis is a common skin disease affecting about 1-3% of the world population. Many types of cells, including lymphocytes, dendritics APCs (antigen presenting cells), NKT (natural killer T) cells, neutrophils, keratinocytes and fibroblasts are involved in the pathogenesis of psoriasis.

The aim of our study was to assess in psoriatic patients the production of IL-8, IL-10 and IL-12 cytokines by neutrophils, fibroblasts and fibroblasts - neutrophils interaction.

Material/Methods: The production of IL-8, IL-10 and IL-12 cytokines was evaluated in supernatants after cells incubation for 21 h in culture medium alone and in medium in the presence of IL-8 or TNF-α. Concentrations of IL-8, IL-10, IL-12 were measured by enzyme-linked immunosorbent assay (ELISA) method using commercially available kits.

Results: Our results demonstrate that fibroblasts are not able to produce IL-10 and IL-12 but they generate IL-8. The amount of IL-8 depends on the site of derivation of fibroblasts and on the stimuli. Neutrophils released IL-8, IL-12 (at a lower level in psoriatic patients) and IL-10 but only in the case of healthy donors and at a very low concentration. Moreover, we observed higher concentrations of IL-12 and IL-8 in supernatants as a result of fibroblasts-neutrophils interaction in psoriatic patients.

Conclusions: Our results suggest that fibroblasts take part in the inflammatory reaction in psoriasis via cytokines or direct interaction with neutrophils. Fibroblasts probably do not exert any anti-inflammatory effect.

Keywords: psoriasis; neutrophils; fibroblasts; cytokines secretion

  • Cimmino MA. Epidemiology of psoriasis and psoriatic arthritis. Reumatismo. 2007 Mar; 59 (Suppl 1):19-24.

  • Büchau AS, Gallo RL. Innate immunity and antimicrobial defense systems in psoriasis. Clin Dermatol. 2007 Nov-Dec;25(6):616-24. [PubMed] [Web of Science] [CrossRef]

  • Bos JD. Psoriasis, innate immunity, and gene pools. J Am Acad Dermatol. 2007 Mar; 56(3):468-71. [CrossRef] [PubMed]

  • Gaspari AA. Innate and adaptive immunity and the pathophysiology of psoriasis. J Am Acad Dermatol. 2006 Mar;54(3 Suppl 2):S67-80. [CrossRef] [Web of Science]

  • De Rosa G, Mignogna C. The histopathology of psoriasis. Reumatismo. 2007 Mar; 59 (Suppl 1):46-8.

  • Loffredo S, Ayala F, Marone GC, Genovese A, Marone G. Immunopathogenesis of psoriasis and psoriatic arthritis and pharmacological perspectives. Reumatismo. 2007 Mar; 59 (Suppl 1):28-39.

  • Boyman O, Curdin C, Tonel G, Gilliet M, Nestle FO. The pathogenic role of tissue-resident immune cells in psoriasis. Trends Immunol. 2007 Feb;28(2):51-7. [CrossRef] [Web of Science] [PubMed]

  • Fredriksson T, Pettersson U. Severe psoriasis-oral therapy with a new retinoid. Dermatologica. 1978;157(4):238-44.

  • Schmitt J, Wozel G. The psoriasis area and severity index is the adequate criterion to define severity in chronic plaque-type psoriasis. Dermatology. 2005;210(3):194-9.

  • Zalewska A, Głowacka E, Wyczółkowska J, Tchórzewski H, Narbutt J, Sysa-Jędrzejowska A. Interleukin 6 and 8 levels in plasma and fibroblast cultures in psoriasis. Mediators Inflamm. 2006;2006(1):81767. [PubMed]

  • Nickoloff BJ, Xin H, Nestle FO, Qin JZ. The cytokine and chemokine network in psoriasis. Clin Dermatol. 2007 Nov-Dec;25(6):568-73. [CrossRef] [PubMed]

  • Victor FC, Gottlieb AB. TNF-alpha and apoptosis: implications for the pathogenesis and treatment of psoriasis. J Drugs Dermatol. 2002 Dec;1(3):264-75.

  • Abanmi A, Al Harthi F, Al Agla R, Khan HA, Tariq M. Serum levels of proinflammatory cytokines in psoriasis patients from Saudi Arabia. Int. J Dermatol. 2005 Jan;44(1):82-3. [CrossRef] [PubMed]

  • Prens EP, Benne K, van Damme J, Bakkus M, Brakel K, Benner R, van Joost T. Interleukin-1 and interleukin-6 in psoriasis. J Invest Dermatol. 1990 Dec;95(6 Suppl):121S-124S.

  • Schröder JM. Peptides and cytokines. Arch Dermatol Res. 1992;284 Suppl 1:S22-26.

  • Mizutani H, Ohmoto Y, Mizutani T, Murata M, Shimizu M. Role of increased production of monocytes TNF-alpha, IL-1beta and IL-6 in psoriasis: relation to focal infection, disease activity and responses to treatments. J Dermatol Sci. 1997 Feb;14(2):145-53.

  • Gordon KB, Ruderman EM. The treatment of psoriasis and psoriatic arthritis: an interdisciplinary approach. J Am Acad Dermatol. 2006 Mar; 54(3 Suppl 2):S85-91. [CrossRef]

  • Buckley CD, Filer A, Haworth O, Parsonage G, Salmon M. Defining a role for fibroblasts in persistence of chronic inflammatory joint disease. Ann Rheum Dis. 2004 Nov;63 Suppl 2:II92-II95.

  • Jordana M, Särnstrand B, Sime PJ, Ramis I. Immune-inflammatory functions of fibroblasts. Eur Respir J. 1994 Dec;7(12):2212-22. [CrossRef] [PubMed]

  • Debets R, Hegmans JP, Buurman WA, Benner R, Prens EP. Expression of cytokines and their receptors by psoriatic fibroblasts. II. decreased TNF receptor expression. Cytokine. 1996 Jan;8(1):80-8. [PubMed] [CrossRef]

  • Miura H, Sanso S, Higashiyama M, Yoshikawa K, Itami S. Involvement of insulin-like growth factor-I in psoriasis as a paracrine growth factor: dermal fibroblasts play a regulatory role in developing psoriatic lesions. Arch Dermatol Res. 2000 Dec;292(12):590-7.

  • Ishikawa H, Mitsuhashi Y, Takagi Y, Hashimoto I. Psoriatic fibroblasts enhance cornified envelope formation in normal keratinocytes in vitro. Arch Dermatol Res. 1997 Aug;289(9):551-3.

  • Cunliffe IA, Richardson PS, Rees RC, Rennie IG. Effect of TNF, IL-1, and IL-6 on proliferation of human Tenon's capsule fibroblasts in tissue culture. Br J Ophthalmol. 1995 Jun;79(6):590-5. [CrossRef]

  • Konstantinova NV, Duong DM, Remenyik E, Hazarika P, Chuang A, Duvic M. Interleukin-8 is induced in skin equivalents and is highest in those derived from psoriatic fibroblast. J Invest Dermatol. 1996 Oct:107(4):615-21.

  • Cicco NA, Lindemann J, Content P, Vandenbussche P, Lübbert M, Gauss J, Mertelsmann R, Herrmann F. Inducible production of interleukin-6 by human polymorphonuclear neutrophils: role of granulocyte-macrophage colony-stimulating factor and tumor necrosis factor-alpha. Blood. 1990 May 15;75(10):2049-52.

  • Dubravec DB, Spriggs DR, Mannick JA, Rodrick ML. Circulating human peripheral blood granulocytes synthesize and secrete tumor necrosis factor alpha. Proc Natl Acad Sci USA. 1990 Sep;87(17):6758-61.

  • Gosselin E, Wardwell K, Rigby WF, Guyre PM. Induction of MHC class II on human polymorphonuclear neutrophils by granulocyte/macrophage colony-stimulating factor, INF-gamma, and IL-3. J Immunol. 1993 Aug 1;151(3):1482-90.

  • Matsukawa A, Yoshinaga M. Neutrophils as a source of cytokine in inflammation. Histol Histopathol. 1999 Apr;14(2):511-6. [PubMed]

  • Duan H, Koga T, Kohda F, Hara H, Urabe K, Furue M. Interleukin-8-positive neutrophils in psoriasis. J Dermatol Sci. 2001 Jun;26(2):119-24.

  • Gillitzer R, Berger R, Mielke V, Müller C, Wolff K, Stingl G. Upper keratinocytes of psoriatic skin lesions express high levels of NAP-1/IL-8mRNA in situ. J Invest Dermatol. 1991 Jul;97(1):73-9. [CrossRef]

  • Debets R, Hegmans JP, Deleuran M, Hooft S, Bennner R, Prens EP. Expression of cytokine and their receptors by psoriatic fibroblasts. I. Altered IL-6 synthesis. Cytokine. 1996 Jan;8(1):71-9.

  • Smith RS, Smith TJ, Blieden TM, Phipps RP. Fibroblasts as sentinel cells. Synthesis of chemokines and regulation of inflammation. Am. J Pathol. 1997 Aug;151(2):317-22.

  • Rosmarin D, Strober BE. The potential of interleukin 12 inhibition in the treatment of psoriasis. J Drugs Dermatol. 2005 May-Jun;4(3):318-25. [PubMed]

  • Asadullah K, Döcke WD, Volk HD, Sterry W. The pathophysiological role of cytokines in psoriasis. Drugs Today (Barc). 1999 Dec;35(12):913-24. [PubMed]

  • Rotsztejn H, Zalewska A, Trznadel-Budźko E, Lewkowicz P, Banasik M, Tchórzewski H. Influence of systemic photochemotherapy on regulatory T cells and selected cytokine productions in psoriatic patients: a pilot study. Med Sci Monit. 2005 Dec; 11(12):CR 594-8.

  • Asadullah K, Sterry W, Stephanek K, Jasulaitis D, Leupold M, Audring H, Volk HD, Döcke WD. IL-10 is a key cytokine in psoriasis. J Clin Invest. 1998 Feb 15;101(4):783-94.

  • Terui T, Ozawa M, Tagami H. Role of neutrophils in induction of acute inflammation in T-cell-mediated immune dermatosis, psoriasis: a neutrophil-asssociated inflammation-boosting loop. Exp Dermatol. 2000 Feb;9(1):1-10. [CrossRef]

Comments (0)

Please log in or register to comment.