Jump to ContentJump to Main Navigation
Show Summary Details

Acta Parasitologica


IMPACT FACTOR increased in 2015: 1.293

SCImago Journal Rank (SJR) 2015: 0.581
Source Normalized Impact per Paper (SNIP) 2015: 0.780
Impact per Publication (IPP) 2015: 1.132

99,00 € / $149.00 / £75.00*

Online
ISSN
1896-1851
See all formats and pricing



Select Volume and Issue
Loading journal volume and issue information...

Fasciola hepatica — the pilot study of in vitro assessing immune response against native and recombinant antigens of the fluke

1Division of Pharmacology and Toxicology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland

2Witold Stefański Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818, Warsaw, Poland

3Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland

4Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences — SGGW, 159C Nowoursynowska Street, 02-776, Warsaw, Poland

© 2013 W. Stefański Institute of Parasitology, PAS. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

Citation Information: Acta Parasitologica. Volume 58, Issue 4, Pages 453–462, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: 10.2478/s11686-013-0163-5, December 2013

Publication History

Published Online:
2013-12-13

Abstract

Fasciola hepatica is a liver fluke that infects 2.4 million of people and causes great economical loss in animal production. To date a 100% effective vaccine has not been developed and the disease is controlled by drug therapy. Great efforts are put into development of effective vaccine against parasite what is difficult since Fasciola spp. (like other helmints) during evolutionary process has developed sophisticated and efficient methods to evade immune response. During preliminary experiments it is convenient to use cell lines which are relatively cheap and allow for reproducible comparison of results between laboratories. We stimulated BOMA (bovine monocyte/macrophage cell line) and BOMAC (bovine macrophage cell line) with native or recombinant antigens of Fasciola hepatica and assessed IFN-γ, IL-4 and TNF-α level upon stimulation. We observed diminished secretion of proinflammatory TNF-α in LPS activated BOMA cells stimulated with Excretory/Secretory products of adult fluke (Fh-ES). We also observed greater changes in gene expression in LPS activated BOMA cells than in non activated BOMA cells upon stimulation using Fh-ES. The results show possibility of using cell lines for in vitro research of bovine immune response against liver fluke, although this model still requires validation and further characterization.

Keywords: Fasciola hepatica; antigens; immune response

  • [1] Aida Y., Pabst M.J. 1990. Removal of endotoxin from protein solutions by phase separation using Triton X-114. Journal of Immunological Methods, 14, 191–195. http://dx.doi.org/10.1016/0022-1759(90)90029-U [CrossRef]

  • [2] Bąska P., Norbury L.J., Wiśniewski M., Januszkiewicz K., Wędrychowicz H. 2013a. Excretory/secretory products of Fasciola hepatica but not recombinant phosphoglycerate kinase induce death of human hepatocyte cells. Acta Parasitologica, 58, 215–217. DOI: 10.2478/s11686-013-0126-x. http://dx.doi.org/10.2478/s11686-013-0126-x [CrossRef] [Web of Science]

  • [3] Bąska P., Wiśniewski M., Krzyżowska M., Długosz E., Zygner W., Górski P., Wędrychowicz H. 2013b. Molecular cloning and characterisation of in vitro immune response against astacinlike metalloprotease Ace-MTP-2 from Ancylostoma ceylanicum. Experimental Parasitology, 133, 472–482. DOI: 10.1016/j.exppara.2013.01.006. http://dx.doi.org/10.1016/j.exppara.2013.01.006 [Web of Science] [CrossRef]

  • [4] Clery D.G., Mulcahy G 1998. Lymphocyte and cytokine responses of young cattle during primary infection with Fasciola hepatica. Research in Veterinary Science, 65,169–171. http://dx.doi.org/10.1016/S0034-5288(98)90171-0 [CrossRef]

  • [5] De Maere V., Vercauteren I., Geldhof P., Gevaert K., Vercruysse J., Claerebout E. 2005. Molecular analysis of astacin-like metalloproteases of Ostertagia ostertagi. Parasitology, 130, 89–98. http://dx.doi.org/10.1017/S0031182004006274 [CrossRef]

  • [6] Donnelly S., O’Neill S.M., Sekiya M., Mulcahy G., Dalton J.P. 2005. Thioredoxin peroxidase secreted by Fasciola hepatica induces the alternative activation of macrophages. Infection and Immunity, 73, 166–173. http://dx.doi.org/10.1128/IAI.73.1.166-173.2005 [CrossRef]

  • [7] Donofrio G., Cavirani S., van Santen V., Flammini C.F. 2005. Potential secondary pathogenic role for bovine herpesvirus 4. Journal of Clinical Microbiology, 43, 3421–3426. http://dx.doi.org/10.1128/JCM.43.7.3421-3426.2005 [CrossRef]

  • [8] Falcón C., Carranza F., Martínez F.F., Knubel C.P., Masih D.T., Motrán C.C., Cervi L. 2010. Excretory-secretory products (ESP) from Fasciola hepatica induce tolerogenic properties in myeloid dendritic cells. Veterinary Immunology Immunopathology, 15, 36–46. DOI: 10.1016/j.vetimm.2010.04.007. http://dx.doi.org/10.1016/j.vetimm.2010.04.007 [Web of Science] [CrossRef]

  • [9] Flynn R.J., Mulcahy G. 2008. Possible role for Toll-like receptors in interaction of Fasciola hepatica excretory/secretory products with bovine macrophages. Infection and Immunity, 76, 678–684. http://dx.doi.org/10.1128/IAI.00732-07 [CrossRef] [Web of Science]

  • [10] Guasconi L., Serradell M.C., Garro A.P., Iacobelli L., Masih D.T. 2011. C-type lectins on macrophages participate in the immunomodulatory response to Fasciola hepatica products. Immunology, 133, 386–396. DOI: 10.1111/j.1365-2567.2011.03449.x. http://dx.doi.org/10.1111/j.1365-2567.2011.03449.x [CrossRef] [Web of Science]

  • [11] Guasconi L., Serradell M.C., Masih D.T. 2012. Fasciola hepatica products induce apoptosis of peritoneal macrophages. Veterinary Immunology and Immunopathology, 148, 359–363. DOI: 10.1016/j.vetimm.2012.06.022. http://dx.doi.org/10.1016/j.vetimm.2012.06.022 [Web of Science] [CrossRef]

  • [12] Jaros S., Jaros D., Wesolowska A., Zygner W., Wedrychowicz H. 2010. Blocking Fasciola hepatica’s energy metabolism — a pilot study of vaccine potential of a novel gene — phosphoglycerate kinase. Veterinary Parasitology, 172, 229–237. DOI: 10.1016/j.vetpar.2010.05.008. http://dx.doi.org/10.1016/j.vetpar.2010.05.008 [Web of Science] [CrossRef]

  • [13] Jolly A., Colavecchia S.B., Fernández B., Fernández E., Mundo S.L. 2011. Antibodies Induced by Lipoarabinomannan in Bovines: Characterization and Effects on the Interaction between Mycobacterium Avium Subsp. paratuberculosis and Macrophages In Vitro. Veterinary Medicine International, 258479. DOI: 10.4061/2011/258479. [CrossRef]

  • [14] Lane G. 1998. Anthelmintic resistance. Veterinary Record, 143, 232.

  • [15] Loukas A., Bethony J.M., Mendez S., Fujiwara R.T., Goud G.N., Ranjit N., Zhan B., Jones K., Bottazzi M.E., Hotez P.J. 2005. Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs. PLoS Medicine. 2, e295. http://dx.doi.org/10.1371/journal.pmed.0020295 [CrossRef]

  • [16] McManus D.P., Dalton J.P. 2006. Vaccines against the zoonotic trematodes Schistosoma japonicum, Fasciola hepatica and Fasciola gigantica. Parasitology, 133 Suppl., 43–61. http://dx.doi.org/10.1017/S0031182006001806 [CrossRef]

  • [17] McVeigh P., Maule A.G., Dalton J.P., Robinson M.W. 2012. Fasciola hepatica virulence-associated cysteine peptidases: a systems biology perspective. Microbes and Infection, 14, 301–310. DOI: 10.1016/j.micinf.2011.11.012. http://dx.doi.org/10.1016/j.micinf.2011.11.012 [Web of Science] [CrossRef]

  • [18] Moll L., Gaasenbeek C.P.H., Vellema P., Borgsteede H.H.M. 2000. Resistance of Fasciola hepatica against triclabendazole in cattle and sheep in The Netherlands. Veterinary Parasitology, 91, 153–158. http://dx.doi.org/10.1016/S0304-4017(00)00267-3 [CrossRef]

  • [19] Norbury L.J., Beckham S., Pike R.N., Grams R., Spithill T.W., Fecondo J.V., Smooker P.M. 2011 Adult and juvenile Fasciola cathepsin L proteases: different enzymes for different roles. Biochimie, 93, 604–611. DOI: 10.1016/j.biochi.2010.12.004. http://dx.doi.org/10.1016/j.biochi.2010.12.004 [Web of Science] [CrossRef]

  • [20] Norbury L.J., Januszkiewicz K., Smooker P. M. 2012. Fasciola: Parasite Biology, Disease and Control. In: (Ed. G.S. Erzinger) Parasites: Ecology, Diseases and Management. Nova Science Publishers, New York, 103–166.

  • [21] Overend D.J, Bowen F.L. 1995. Resistance of Fasciola hepatica to triclabendazole. Australian Veterinary Journal, 72, 275–276. http://dx.doi.org/10.1111/j.1751-0813.1995.tb03546.x [CrossRef]

  • [22] Pearson M.S., Bethony J.M., Pickering D.A., de Oliveira L.M., Jariwala A., Santiago H., Miles A.P., Zhan B., Jiang D., Ranjit N., Mulvenna J., Tribolet L., Plieskatt J., Smith T., Bottazzi M.E., Jones K., Keegan B., Hotez P.J., Loukas A. 2009. An enzymatically inactivated hemoglobinase from Necator americanus induces neutralizing antibodies against multiple hookworm species and protects dogs against heterologous hookworm infection. FASEB Journal, 23, 3007–3019. DOI: 10.1096/fj.09-131433. http://dx.doi.org/10.1096/fj.09-131433 [Web of Science] [CrossRef]

  • [23] Pearson M.S., Pickering D.A., Tribolet L., Cooper L., Mulvenna J., Oliveira L.M., Bethony J.M,. Hotez P.J., Loukas A. 2010. Neutralizing antibodies to the hookworm hemoglobinase Na-APR-1: implications for a multivalent vaccine against hookworm infection and schistosomiasis. Journal of Infectious Diseases, 15,201(10), 1561–1569. DOI: 10.1086/651953. http://dx.doi.org/10.1086/651953 [CrossRef] [Web of Science]

  • [24] Ranjit N., Zhan B., Hamilton B., Stenzel D., Lowther J., Pearson M., Gorman J., Hotez P., Loukas A. 2009. Proteolytic degradation of hemoglobin in the intestine of the human hookworm Necator americanus. Journal of Infectious Diseases, 199, 904–912. http://dx.doi.org/10.1086/597048 [Web of Science] [CrossRef]

  • [25] Rola M., Materniak M., Pluta A., Kuzmak J. 2011. DNA microarray gene expression profile of bovine macrophages cell line (BoMac) after infection with Bovine Immunodeficiency Virus or Bovine Foamy Virus. Retrovirology, 8(Suppl. 1), A22. DOI: 10.1186/1742-4690-8-S1-A22. http://dx.doi.org/10.1186/1742-4690-8-S1-A22 [CrossRef]

  • [26] Salazar-Calderón M., Martín-Alonso J.M., Ruiz de Eguino A.D., Casais R., Marin M.S., Parra F. 2000. Fasciola hepatica: Heterologous expression and functional characterization of Thioredoxin Peroxidase. Experimental Parasitology, 95, 63–70. http://dx.doi.org/10.1006/expr.2000.4495 [CrossRef]

  • [27] Schweizer G., Braun U., Deplazes P., Torgerson P.R. 2005. Estimating the financial losses due to bovine fasciolosis in Switzerland. Veterinary Records, 157, 188–193.

  • [28] Serradell M.C., Guasconi L., Masih D.T. 2009. Involvement of a mitochondrial pathway and key role of hydrogen peroxide during eosinophil apoptosis induced by excretory-secretory products from Fasciola hepatica. Molecular and Biochemical Parasitology,163, 95–106. DOI: 10.1016/j.molbiopara.2008.10.005. http://dx.doi.org/10.1016/j.molbiopara.2008.10.005 [CrossRef] [Web of Science]

  • [29] Shiels B.R., McKellar S., Katzer F., Lyons K., Kinnaird J., Ward C., Wastling J.M., Swan D. 2004. A Theileria annulata DNA binding protein localized to the host cell nucleus alters the phenotype of a bovine macrophage cell line. Eukaryotic Cell, 3, 495–505. http://dx.doi.org/10.1128/EC.3.2.495-505.2004 [CrossRef]

  • [30] Sreekrishna K., Potenz R.H., Cruze J.A., McCombie W.R., Parker K.A,. Nelles L., Mazzaferro P.K., Holden K.A., Harrison R.G., Wood P.J, et al. 1988. High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris. Journal of Basic Microbiology. 28, 265–278. http://dx.doi.org/10.1002/jobm.3620280410 [CrossRef]

  • [31] Stabel J.R., Stabel T.J. 1995. Immortalization and characterization of bovine peritoneal macrophages transfected with SV40 plasmid DNA. Veterinary Immunology and Immunopathology, 45, 211–220. http://dx.doi.org/10.1016/0165-2427(94)05348-V [CrossRef]

  • [32] van Riet E., Hartgers F.C., Yazdanbakhsh M. 2007. Chronic helminth infections induce immunomodulation: consequences and mechanisms. Immunobiology, 212, 475–490. DOI: 10.1016/j.imbio.2007.03.009 http://dx.doi.org/10.1016/j.imbio.2007.03.009 [Web of Science] [CrossRef]

  • [33] Wasyl K., Zawistowska-Deniziak A., Bąska P., Wędrychowicz H., Wiśniewski M. 2013. Molecular cloning and expression of the cDNA sequence encoding a novel aspartic protease from Uncinaria stenocephala. Experimental Parasitology, 134, 220–227. DOI: 10.1016/j.exppara.2013.03.013. http://dx.doi.org/10.1016/j.exppara.2013.03.013 [CrossRef] [Web of Science]

  • [34] Weaver C.T., Harrington L.E., Mangan P.R., Gavrieli M., Murphy KM. 2006. Th17: an effector CD4 T cell lineage with regulatory T cell ties. Immunity, 24, 677–688. Review. http://dx.doi.org/10.1016/j.immuni.2006.06.002 [CrossRef]

  • [35] Wędrychowicz H., Klockiewicz M. 1994. Protective and diagnostic molecules of Fasciola hepatica. Acta Parasitologica, 39, 173–178.

  • [36] Williamson A.L., Brindley P.J., Loukas A. 2003. Hookworm cathepsin D aspartic proteases: contributing roles in the host-specific degradation of serum proteins and skin macromolecules. Parasitology, 126, 179–185. http://dx.doi.org/10.1017/S0031182002002706 [CrossRef]

  • [37] Williamson A.L., Lecchi P., Turk B.E., Choe Y., Hotez P.J., McKerrow J.H., Cantley L.C., Sajid M., Craik C.S., Loukas A. 2004. A multi-enzyme cascade of hemoglobin proteolysis in the intestine of blood-feeding hookworms. Journal of Biological Chemistry, 279, 35950–35957. http://dx.doi.org/10.1074/jbc.M405842200 [CrossRef]

  • [38] Wilson L.R., Good R.T., Panaccio M., Wijffels G.L., Sandeman R.M., Spithill T.W. 1998. Fasciola hepatica: characterization and cloning of the major cathepsin B protease secreted by newly excysted juvenile liver fluke. Experimental Parasitology, 88,85–94. http://dx.doi.org/10.1006/expr.1998.4234 [CrossRef]

  • [39] Zawistowska-Deniziak A., Wasyl K., Norbury L.J., Wesołowska A., Bień J., Grodzik M., Wiśniewski M., Bąska P., Wędrychowicz H. 2013. Characterization and differential expression of cathepsin L3 alleles from Fasciola hepatica. Molecular and Biochemical Parasitology, 190, 27–37. DOI: 10.1016/j.molbiopara.2013.06.001. http://dx.doi.org/10.1016/j.molbiopara.2013.06.001 [CrossRef]

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Ewa Długosz and Marcin Wiśniewski
Acta Parasitologica, 2016, Volume 61, Number 1
[2]
Matheus D. Baldissera, Nathieli B. Bottari, Ricardo E. Mendes, Claiton I. Schwertz, Neuber J. Lucca, Diessica Dalenogare, Guilherme V. Bochi, Rafael N. Moresco, Vera M. Morsch, Maria R.C. Schetinger, Virginia C. Rech, Jeandre A. Jaques, and Aleksandro S. Da Silva
Pathology - Research and Practice, 2015
[3]
Nathieli B. Bottari, Ricardo E. Mendes, Neuber J. Lucca, Claiton I. Schwertz, Luan C. Henker, Débora C. Olsson, Manoela M. Piva, Manuela Sangoi, Luízi P. Campos, Rafael N. Moresco, Jeandre A. Jaques, and Aleksandro S. Da silva
Experimental Parasitology, 2015

Comments (0)

Please log in or register to comment.