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Publication Date:
June 2005
ISSN:
1437-4315
DOI:
10.1515/BC.1999.013

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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Ludwig, Stephan / Sies, Helmut / Stoffel, Markus / Turk, Boris / Wittinghofer, Alfred / Baumeister, Wolfgang / Bergeron, John / Bogyo, Matthew / Bürkle, Alexander / Cadenas, Enrique / Chiti, Fabrizio / Dikic, Ivan / Dobson, Christopher / Driessen, Arnold / Fritz, Hans / Gevaert, Kris / Hammann, Christian / Hartl, F. Ulrich / Häussinger, Dieter / Hiscott, John / Igarashi, Yasuyuki / Klotz, Lars-Oliver / Krüger, Achim / Magdolen, Viktor / Müschen, Markus / Narumiya, Shuh / Naumann, Michael / Pejler, Gunnar / Pfanner, Nikolaus / Pike, Robert / Potempa, Jan / Saftig, Paul / Sandhoff, Konrad / Schaffner, Walter / Sinning, Irmgard / Sommerhoff, Christian P.

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cDNA Sequencing of Guinea Pig a2-HS Glycoprotein, Its Expression in Various Tissues and Acute Phase Expression

K. Yoshida / Y. Suzuki / K. Yamamoto / H. Sinohara

Citation Information: Biological Chemistry. Volume 380, Issue 1, Pages 95–99, ISSN (Print) 1431-6730, DOI: 10.1515/BC.1999.013, June 2005

Publication History:
Published Online:
2005-06-01

Abstract

cDNA encoding α2-HS glycoprotein was amplified from guinea pig liver mRNA by reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends, cloned and sequenced. By RTPCR and nested PCR, α2-HS glycoprotein mRNA was detected not only in liver tissue but also in pancreas, stomach, small intestine, colon, spleen, kidney, testis, skeletal muscle, brain, heart and leukocytes, but not in the lung. The α2-HS glycoprotein mRNA levels in the liver were reduced to half at 48 h after subcutaneous injection of turpentine oil.

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