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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

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IMPACT FACTOR increased in 2014: 3.268
Rank 106 out of 289 in category Biochemistry & Molecular Biology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

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On the Role of Thymopoietins in Cell Proliferation. Immunochemical Evidence for New Members of the Human Thymopoietin Family

P.J.A. Weber / C.P. Eckard / S. Gonser / H. Otto / G. Folkers / A.G. Beck-Sickinger

Citation Information: Biological Chemistry. Volume 380, Issue 6, Pages 653–660, ISSN (Print) 1431-6730, DOI: 10.1515/BC.1999.081, June 2005

Publication History

Published Online:
2005-06-01

Abstract

Thymopoietins (TMPOs) are a group of ubiquitously expressed nuclear proteins. They are suggested to play an important role in nuclear envelope organization and cell cycle control, as has been shown for lamina-associated polypeptides 2 α and β, which are the rat homologs of human TMPOα and TMPOβ, respectively. The recent isolation and characterization of seven mouse TMPO mRNA transcripts named TMPO-α, β, β', γ, ε, δ, and ζ, suggest that more than the three previously reported transcripts, α, β, and γ forms, may exist in humans. Here we report on the demonstration of putative human TMPOδ and ε by immunoblotting of human cell lines using a newly prepared polyclonal antiserum against the common N-terminal region of TMPO. Furthermore, we prepared the first truly TMPO-β-specific, affinity-purified polyclonal antiserum, using a part of the human analog of the β-specific domain of mouse TMPO 220–259 for immunization. We showed that human TMPOβ is highly expressed in all cancerous cells tested, while hardly any cross-reactivities with other proteins could be detected. In contrast to the high expression of human TMPOβ in the cancer-derived neuroblastoma cell lines SK-N-MC and SMS-KAN, we found very low expression of human TMPOβ in low-proliferative nerve tissue. These data led us to the assumption that expression of TMPOβ may correlate with the occurrence of cancer, and therefore may serve as a new tumor marker, or even as a new target for cancer therapy.

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[2]
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