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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

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Phage Display of RNase A and an Improved Method for Purification of Phages Displaying RNases

Kerstin Korn / Hans-Heinrich Foerster / Ulrich Hahn

Citation Information: Biological Chemistry. Volume 381, Issue 2, Pages 179–181, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2000.024, June 2005

Publication History

Published Online:
2005-06-01

Abstract

Functional ribonuclease A was presented on the surface of the filamentous phage M13 by fusion to the minor coat protein. RNase activity of the fusion protein was shown by a zymogram assay. In addition, we established a modified method for preparing RNasedisplaying phages without contaminating host RNases.

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