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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

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Splice Variants of Human Cathepsin L mRNA Show Different Expression Rates

Abulizi Abudula / Winfried Rommerskirch / Ekkehard Weber / Dagmar Günther / Bernd Wiederanders

Citation Information: Biological Chemistry. Volume 382, Issue 11, Pages 1583–1591, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2001.193, June 2005

Publication History

Published Online:
2005-06-01

Abstract

Human cathepsin L (hCATL) mRNA occurs in vivo in at least three splice variants. They differ in the length of exon 1, which comprises 278 nucleotides (hCATLA), 188 nucleotides (hCATLA2) and 132 nucleotides (hCATLA3), respectively. We describe here the shortest variant for the first time. This form is predominant in all tissues and cells examined so far, including malignant tumors. We studied the expression rate of the three mRNA variants in order to explain why malignant kidney tumors show low cathepsin L activity despite of high mRNA levels. The variant hCATLA3 showed the highest expression rate in vitro and in vivo. Based on these results, we suggest a cisacting element on human cathepsin L mRNA which can be bound by a negative transacting regulator, thus leading to reduced expression rates.

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