Jump to ContentJump to Main Navigation

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR increased in 2014: 3.268
Rank 106 out of 289 in category Biochemistry & Molecular Biology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2014: 1.596
Source Normalized Impact per Paper (SNIP) 2014: 0.845
Impact per Publication (IPP) 2014: 2.992



Identification and Characterization of Proteins That Selectively Interact with Isoforms of the mRNA Binding Protein AUF1 (hnRNP D)

K.C.M. Moraes / A.J.C. Quaresma / K. Maehnss / J. Kobarg

Citation Information: Biological Chemistry. Volume 384, Issue 1, Pages 25–37, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2003.004, June 2005

Publication History

Published Online:


The mRNAs that encode certain cytokines and proto-oncogenes frequently contain a typical AUrich motif that is located in their 3'-untranslated region. The protein AUF1 is the first factor identified that binds to AU rich regions and mediates the fast degradation of the target mRNAs. AUF1 exists as four different isoforms (p37, p40, p42 and p45) that are generated by alternative splicing. The fact that AUF1 does not degrade mRNA itself had led to the suggestion that other AUF1 interacting proteins might be involved in the process of selective mRNA degradation. Here we used the yeast two-hybrid system in order to identify proteins that bind to AUF1. We detected AUF1 itself, as well as the ubiquitin-conjugating enzyme E2I and three RNA binding proteins: NSEP-1, NSAP-1 and IMP-2, as AUF1 interacting proteins. We confirmed all interactions in vitro and mapped the protein domains that are involved in the interaction with AUF1. Gelshift assays with the recombinant purified proteins suggest that the interacting proteins and AUF1 can bind simultaneously to an AU-rich RNA oligonucleotide. Most interestingly, the AUF1 interacting protein NSEP-1showed an endoribonuclease activity in vitro. These data suggest the possibility that the identified AUF1 interacting proteins might be involved in the regulation of mRNA stability mediated by AUF1.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Kevin Czaplinski, Thomas Köcher, Malgorzata Schelder, Alexandra Segref, Matthias Wilm, and Iain W. Mattaj
Developmental Cell, 2005, Volume 8, Number 4, Page 505
Lei Wang, Thippeswamy Gulappa, and K.M.J. Menon
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2010, Volume 1803, Number 5, Page 591
Sebastiaan van Zalen, Alyssa A. Lombardi, Grace R. Jeschke, Elizabeth O. Hexner, and J. Eric Russell
Mechanisms of Development, 2015, Volume 136, Page 40
Selena M. Sagan, Jasmin Chahal, and Peter Sarnow
Virus Research, 2015, Volume 206, Page 90
D. N. Lyabin, L. F. Nigmatullina, A. N. Doronin, I. A. Eliseeva, and L. P. Ovchinnikov
Biochemistry (Moscow), 2013, Volume 78, Number 6, Page 651
Pavel Ivanov and Paul Anderson
Immunological Reviews, 2013, Volume 253, Number 1, Page 253
Joel K. Yisraeli
Biology of the Cell, 2005, Volume 97, Number 1, Page 87
I. A. Eliseeva, E. R. Kim, S. G. Guryanov, L. P. Ovchinnikov, and D. N. Lyabin
Biochemistry (Moscow), 2011, Volume 76, Number 13, Page 1402
Markus H. Hoffmann, Sylvie Trembleau, Sylviane Muller, and Günter Steiner
Journal of Autoimmunity, 2010, Volume 34, Number 3, Page J178
Alexandre J.C. Quaresma, Sergio Oyama, João A.R.G. Barbosa, and Jörg Kobarg
Biochemical and Biophysical Research Communications, 2006, Volume 350, Number 2, Page 288
Dario O. Passos, Alexandre J.C. Quaresma, and Jörg Kobarg
Biochemical and Biophysical Research Communications, 2006, Volume 346, Number 2, Page 517
Flávia C. Nery, Edmilson Rui, Taís M. Kuniyoshi, and Jörg Kobarg
Biochemical and Biophysical Research Communications, 2006, Volume 341, Number 3, Page 847
Patricia Ribeiro de Moura, Edmilson Rui, Kaliandra de Almeida Gonçalves, and Jörg Kobarg
Virus Research, 2005, Volume 108, Number 1-2, Page 121
Changlu Li, Xiuli Wang, Gengyu Wang, Changxin Wu, and Ning Li
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2011, Volume 6, Number 3, Page 264
Bogusz Trojanowicz, Henning Dralle, and Cuong Hoang-Vu
Thyroid Research, 2011, Volume 4, Number Suppl 1, Page S5
Alexandre J.C. Quaresma, G.C. Bressan, L.M. Gava, D.C.F. Lanza, C.H.I Ramos, and Jörg Kobarg
Experimental Cell Research, 2009, Volume 315, Number 6, Page 968
Khalid S.A. Khabar
Journal of Interferon & Cytokine Research, 2005, Volume 25, Number 1, Page 1
Gary Brewer and Sandra Chesoni
AfCS-Nature Molecule Pages, 2006
Frances M. Gratacós and Gary Brewer
Wiley Interdisciplinary Reviews: RNA, 2010, Volume 1, Number 3, Page 457
Dario O. Passos, Gustavo C. Bressan, Flavia C. Nery, and Jörg Kobarg
FEBS Journal, 2006, Volume 273, Number 17, Page 3946
Gustavo C. Bressan, Alexandre J. C. Quaresma, Eduardo C. Moraes, Adriana O. Manfiolli, Dario O. Passos, Marcelo D. Gomes, and Jörg Kobarg
FEBS Journal, 2009, Volume 276, Number 14, Page 3770
Karl Skriner, Wolfgang Hueber, Erhan Süleymanoglu, Elisabeth Höfler, Veit Krenn, Josef Smolen, and Günter Steiner
Arthritis & Rheumatism, 2008, Volume 58, Number 2, Page 511

Comments (1)

Please log in or register to comment.

    posted by: dominika hrossova on 2012-06-18 04:02 PM (Europe/Berlin)