Immunogenicity and Protectivity of Plasmodium falciparum EBA-175 Peptide and Its Analog Is Associated with α-Helical Region Shortening and Displacement : Biological Chemistry

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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred


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Immunogenicity and Protectivity of Plasmodium falciparum EBA-175 Peptide and Its Analog Is Associated with α-Helical Region Shortening and Displacement

A. Bermúdez / G. Cifuentes / F. Guzmán / L. M. Salazar / M. E. Patarroyo

Citation Information: Biological Chemistry. Volume 384, Issue 10-11, Pages 1443–1450, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2003.160, June 2005

Publication History

Published Online:
2005-06-01

Abstract

EBA-175 protein is used as a ligand in the binding of P. falciparum to red blood cells (RBCs). Evidence shows that the conserved peptide 1779 from this protein (with high red blood cell binding ability and known critical erythrocyte binding residues) plays an important role in the invasion process. This peptide is neither immunogenic nor protective; analogs having critical residues replaced by amino acids with similar volume or mass but different polarity were synthesized and inoculated into Aotus monkeys, and elicited different immunogenic and protective responses. Nuclear Magnetic Resonance (1H-NMR) studies revealed that peptide analog 21696 (non-immunogenic and non-protective) presents a large helical fragment, that the peptide 14012 (immunogenic and non-protective) helical fragment is smaller, while the peptide 22812 (immunogenic and protective) α-helix is shorter in a different region and possesses greater flexibility at its N-terminus. The presence of methionine residues could affect the structural stability of peptide 22812 and ultimately its immunological response. Our results suggest a new strategy for designing a new malaria multi-component subunit-based vaccine.

Citing Articles

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[1]
Adriana Bermúdez, Martha Patricia Alba, Magnolia Vanegas, and Manuel Elkin Patarroyo
Vaccine, 2010, Volume 28, Number 31, Page 4989
[3]
M. E. Patarroyo, G. Cifuentes, A. Bermúdez, and M. A. Patarroyo
Journal of Cellular and Molecular Medicine, 2008, Volume 12, Number 5b, Page 1915
[4]
Jeison García, Hernando Curtidor, Olga L. Gil, Magnolia Vanegas, and Manuel E. Patarroyo
Biochemical and Biophysical Research Communications, 2009, Volume 380, Number 1, Page 122
[5]
Marisol Ocampo, Hernando Curtidor, Ricardo Vera, John J Valbuena, Luis E Rodrı́guez, Álvaro Puentes, Ramses López, Javier E Garcı́a, Diana Tovar, Paola Pacheco, Miguel A Navarro, and Manuel E Patarroyo
Biochemical and Biophysical Research Communications, 2004, Volume 315, Number 2, Page 319
[6]
Martha Patricia Alba, Luz Mary Salazar, Luis Eduardo Vargas, Mary Trujillo, Yolanda Lopez, and Manuel Elkin Patarroyo
Biochemical and Biophysical Research Communications, 2004, Volume 315, Number 4, Page 1154
[7]
Ramsés López, John Valbuena, Luis E. Rodríguez, Marisol Ocampo, Ricardo Vera, Hernando Curtidor, Alvaro Puentes, Javier García, Luis E. Ramirez, and Manuel E. Patarroyo
Peptides, 2006, Volume 27, Number 7, Page 1685
[9]
Fabiola Espejo, Adriana Bermúdez, Magnolia Vanegas, Zuly Rivera, Elizabeth Torres, Luz Mary Salazar, and Manuel Elkin Patarroyo
Journal of Structural Biology, 2005, Volume 150, Number 3, Page 245
[10]
Jeison García, Hernando Curtidor, Carlos G. Pinzón, Magnolia Vanegas, Armando Moreno, and Manuel E. Patarroyo
Vaccine, 2009, Volume 27, Number 30, Page 3953
[11]
Hernando Curtidor, Jeison García, Magnolia Vanegas, Fabian Puentes, Martha Forero, and Manuel Elkin Patarroyo
Biochimie, 2008, Volume 90, Number 11-12, Page 1750
[12]
Manuel E. Patarroyo, Gladys Cifuentes, Nora L. Martínez, and Manuel A. Patarroyo
Progress in Biophysics and Molecular Biology, 2010, Volume 102, Number 1, Page 38
[13]
Manuel Elkin Patarroyo, Adriana Bermúdez, and Manuel Alfonso Patarroyo
Chemical Reviews, 2011, Volume 111, Number 5, Page 3459
[14]
Adriana Bermúdez, Patricia Alba, Fabiola Espejo, Luis EduardoVargas, Carlos Parra, Raúl Rodríguez, Claudia Reyes, and Manuel Elkin Patarroyo
The International Journal of Biochemistry & Cell Biology, 2005, Volume 37, Number 2, Page 336
[15]
Hernando Curtidor, Mary Helena Torres, Martha Patricia Alba, and Manuel E. Patarroyo
Biological Chemistry, 2007, Volume 388, Number 1

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