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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred


SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

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1437-4315
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Recruitment of the Interleukin-1 Receptor (IL-1RI)-Associated Kinase IRAK to the IL-1RI Is Redox Regulated

G.-F. Böl / N. Jurrmann / R. Brigelius-Flohé

Citation Information: Biological Chemistry. Volume 384, Issue 4, Pages 609–617, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2003.068, June 2005

Publication History

Published Online:
2005-06-01

Abstract

Interleukin-1 signaling is initiated by recruitment of adapter proteins and kinases to the type I interleukin-1 receptor (IL-1RI). It is modulated by accompanying redox processes at various levels, such as (auto-) phosphorylation of the IL-1RIassociated kinase IRAK, the phosphorylation of IκB and translocation and transcriptional activity of NFκB. Here we demonstrate that the thiolmodifying agents diamide, menadione, and phenylarsine oxide (PAO) block the recruitment of IRAK to the receptor without inhibiting kinase activity in the immunoprecipitated IL-1RI complex in the human epithelial cell line ECV304 and the murine T cell line EL-4. Inhibition of IRAK receptor association by menadione is reversible in a GSH-dependent manner, while the PAO effect proved to be irreversible. Phospholipid hydroperoxide glutathione peroxidase attenuates inhibition by menadione. Recruitment correlates with the presence of thiol groups in IRAK that were available for IAIT-labeling. We conclude that recruitment of IRAK to the IL-1RI is redox regulated by the glutathione system, a reduced status being a prerequisite for an appropiate IL-1 response.

Citing Articles

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Juliane Hrdina, Antje Banning, Anna Kipp, Gunnar Loh, Michael Blaut, and Regina Brigelius-Flohé
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[2]
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[3]
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[4]
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[5]
Gaby-Fleur Böl, Nadine Jurrmann, and Regina Brigelius-Flohé
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[6]
Dirk Kluth, Antje Banning, Ingvild Paur, Rune Blomhoff, and Regina Brigelius-Flohé
Free Radical Biology and Medicine, 2007, Volume 42, Number 3, Page 315
[7]
Ulrich Schweizer, Anja U Bräuer, Josef Köhrle, Robert Nitsch, and Nicolai E Savaskan
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[8]
Sergio Rosales-Corral, Russel J. Reiter, Dun-Xian Tan, Genaro G. Ortiz, and Gabriela Lopez-Armas
Antioxidants & Redox Signaling, 2010, Volume 13, Number 2, Page 193
[9]
Antje Banning and Regina Brigelius-Flohé
Antioxidants & Redox Signaling, 2005, Volume 7, Number 7-8, Page 889
[10]
Jana Hemmerling, Sandra Nell, Anna Kipp, Sara Schumann, Stefanie Deubel, Michael Haack, and Regina Brigelius-Flohé
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