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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


SCImago Journal Rank (SJR): 1.550
Source Normalized Impact per Paper (SNIP): 0.734

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An extracellular carboxylesterase from the basidiomycete Pleurotus sapidus hydrolyses xanthophyll esters

Holger Zorn1 / Henning Bouws2 / Meike Takenberg3 / Manfred Nimtz4 / Rita Getzlaff5 / Dietmar E. Breithaupt6 / Ralf G. Berger7

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Corresponding author

Citation Information: Biological Chemistry. Volume 386, Issue 5, Pages 435–440, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2005.052, July 2005

Publication History

Received:
February 9, 2005
Accepted:
March 22, 2005
Published Online:
2005-07-05

Abstract

An extracellular enzyme capable of efficient hydrolysis of xanthophyll esters was purified from culture supernatants of the basidiomycete Pleurotus sapidus. Under native conditions, the enzyme exhibited a molecular mass of 430 kDa, and SDS-PAGE data suggested a composition of eight identical subunits. Biochemical characterisation of the purified protein showed an isoelectric point of 4.5, and ideal hydrolysis conditions were observed at pH 5.8 and 40°C. Partial amino acid sequences were derived from N-terminal Edman degradation and from mass spectrometric ab initio sequencing of internal peptides. An 1861-bp cDNA containing an open reading frame of 1641 bp was cloned from a cDNA library that showed ca. 40% homology to Candida rugosa lipases. The P. sapidus carboxylesterase represents the first enzyme of the lipase/esterase family from a basidiomycetous fungus that has been characterised at the molecular level.

Keywords: carotenoids; cDNA library; fungi; lipase

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