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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

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Characterisation of YtfM, a second member of the Omp85 family in Escherichia coli

Johannes F. Stegmeier1 / Andreas Glück2 / Suja Sukumaran3 / Werner Mäntele4 / Christian Andersen5

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Corresponding author

Citation Information: Biological Chemistry. Volume 388, Issue 1, Pages 37–46, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2007.004, January 2007

Publication History

Received:
May 17, 2006
Accepted:
August 28, 2006
Published Online:
2007-01-10

Abstract

Omp85 proteins form a ubiquitous protein family, members of which are found in all Gram-negative bacteria. Omp85 of Neisseria meningitidis and YaeT of Escherichia coli are shown to be essential for outer membrane biogenesis. Interestingly, there exists a homologue to YaeT in E. coli and many proteobacteria, denoted YtfM, the function of which has not been described yet. Like YaeT, YtfM is predicted to consist of an amino-terminal periplasmic domain and a membrane-located carboxy-terminal domain. In this study, we present a first characterisation of YtfM by comparison to YaeT concerning structural, biochemical and electrophysiological properties. Furthermore, a knockout strain revealed that ytfM is a non-essential gene and lack of the protein had no effect on outer membrane composition and integrity. The only observable phenotype was strongly reduced growth, indicating an important role of YtfM in vivo.

Keywords: black lipid membranes; CD and FTIR spectroscopy; outer membrane protein; pore-forming protein; structure analysis; YaeT

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