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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred


SCImago Journal Rank (SJR) 2015: 1.607
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Impact per Publication (IPP) 2015: 2.609

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1437-4315
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Idiosyncratic cleavage and ligation activity of individual hammerhead ribozymes and core sequence variants thereof

Rita Przybilski1 / Christian Hammann2

1AG Molecular Interactions, Department of Genetics, University of Kassel, Heinrich-Plett-Str. 40, D-34132 Kassel, Germany

2AG Molecular Interactions, Department of Genetics, University of Kassel, Heinrich-Plett-Str. 40, D-34132 Kassel, Germany

Corresponding author

Citation Information: Biological Chemistry. Volume 388, Issue 7, Pages 737–741, ISSN (Online) 14316730, ISSN (Print) 14374315, DOI: 10.1515/BC.2007.065, July 2007

Publication History

Received:
2007-02-13
Accepted:
2007-03-02
Published Online:
2007-07-01

Abstract

The hammerhead ribozyme is a small RNA endonuclease found in sub-viral plant pathogens, in transcripts from certain animal satellite DNAs and encoded at distinct loci of Arabidopsis thaliana. Kinetic analyses of tertiary stabilised ribozymes from peach latent mosaic viroid (PLMVd), Schistosoma mansoni and A. thaliana revealed a ten-fold difference in cleavage rates. Core nucleotide variations affected cleavage reactions least in the A. thaliana ribozyme, and most in the S. mansoni ribozyme. The reverse ligation reaction was catalysed efficiently by the PLMVd and A. thaliana ribozymes. The different behaviour of the individual hammerhead ribozymes is discussed in terms of structure and function.

Keywords: catalytic RNA; divalent metal ions; RNA structure; satellite DNA; viroid

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