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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

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Rank 106 out of 289 in category Biochemistry & Molecular Biology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

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Regulation of the expression of components of the exocytotic machinery of insulin-secreting cells by microRNAs

Pascal Lovis1 / Sonia Gattesco2 / Romano Regazzi3

1Department of Cell Biology and Morphology, Faculty of Biology and Medicine, University of Lausanne, CH-1005 Lausanne, Switzerland

2Department of Cell Biology and Morphology, Faculty of Biology and Medicine, University of Lausanne, CH-1005 Lausanne, Switzerland

3Department of Cell Biology and Morphology, Faculty of Biology and Medicine, University of Lausanne, CH-1005 Lausanne, Switzerland

Corresponding author

Citation Information: Biological Chemistry. Volume 389, Issue 3, Pages 305–312, ISSN (Online) 14374315, ISSN (Print) 14316730, DOI: 10.1515/BC.2008.026, March 2008

Publication History

Received:
2007-08-03
Accepted:
2007-11-27
Published Online:
2008-03-03

Abstract

Fine-tuning of insulin secretion from pancreatic β-cells participates in blood glucose homeostasis. Defects in this process can lead to chronic hyperglycemia and diabetes mellitus. Several proteins controlling insulin exocytosis have been identified, but the mechanisms regulating their expression remain poorly understood. Here, we show that two non-coding microRNAs, miR124a and miR96, modulate the expression of proteins involved in insulin exocytosis and affect secretion of the β-cell line MIN6B1. miR124a increases the levels of SNAP25, Rab3A and synapsin-1A and decreases those of Rab27A and Noc2. Inhibition of Rab27A expression is mediated by direct binding to the 3′-untranslated region of Rab27A mRNA. The effect on the other genes is indirect and linked to changes in mRNA levels. Over-expression of miR124a leads to exaggerated hormone release under basal conditions and a reduction in glucose-induced secretion. miR96 increases mRNA and protein levels of granuphilin, a negative modulator of insulin exocytosis, and decreases the expression of Noc2, resulting in lower capacity of MIN6B1 cells to respond to secretagogues. Our data identify miR124a and miR96 as novel regulators of the expression of proteins playing a critical role in insulin exocytosis and in the release of other hormones and neurotransmitters.

Keywords: exocytosis; insulin secretion; microRNA; neurotransmitter release; pancreatic β-cell

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