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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

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Human CYP4Z1 catalyzes the in-chain hydroxylation of lauric acid and myristic acid

Andy Zöllner1 / Calin-Aurel Dragan2 / Dominik Pistorius3 / Rolf Müller4 / Helge B. Bode5 / Frank T. Peters6 / Hans H. Maurer7 / Matthias Bureik8

1PomBioTech GmbH, Campus Geb. A1-1, D-66123 Saarbrücken, Germany

2PomBioTech GmbH, Campus Geb. A1-1, D-66123 Saarbrücken, Germany

3Department of Pharmaceutical Biotechnology, Saarland University, D-66041 Saarbrücken, Germany

4Department of Pharmaceutical Biotechnology, Saarland University, D-66041 Saarbrücken, Germany

5Department of Pharmaceutical Biotechnology, Saarland University, D-66041 Saarbrücken, Germany

6Department of Experimental and Clinical Toxicology, Saarland University, D-66421 Homburg (Saar), Germany

7Department of Experimental and Clinical Toxicology, Saarland University, D-66421 Homburg (Saar), Germany

8PomBioTech GmbH, Campus Geb. A1-1, D-66123 Saarbrücken, Germany

Corresponding author

Citation Information: Biological Chemistry. Volume 390, Issue 4, Pages 313–317, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2009.030, December 2008

Publication History

Received:
2008-11-21
Accepted:
2008-12-03
Published Online:
2008-12-17

Abstract

Overexpression of human CYP4Z1, a cytochrome P450 enzyme, has been correlated with poor prognosis in human cancer. However, its catalytic properties are not yet known. We expressed this P450 in Schizosaccharomyces pombe and demonstrate by whole-cell biotransformation assays CYP4Z1-dependent in-chain hydroxylation of lauric and myristic acid, which in both cases leads to the formation of four different monohydroxylated products at positions ω-2, ω-3, ω-4, and ω-5, respectively. The CYP4Z1-expressing fission yeast should be a new valuable tool for testing cancer drugs or for the development of new prodrug strategies.

Keywords: breast cancer; cancer treatment; cytochrome P450; fatty acid hydroxylation; Schizosaccharomyces pombe; whole-cell biotransformation

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