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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

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Staphylococcal cysteine protease staphopain B (SspB) induces rapid engulfment of human neutrophils and monocytes by macrophages

Jan Smagur1 / Krzysztof Guzik2 / Malgorzata Bzowska3 / Mateusz Kuzak4 / Miroslaw Zarebski5 / Tomasz Kantyka6 / Michal Walski7 / Barbara Gajkowska8 / Jan Potempa9

1Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland

2Department of Immunology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland

3Department of Immunology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland

4Department of Cell Biophysics, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland

5Department of Cell Biophysics, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland

6Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland

7Department of Cell Ultrastructure, Mossakowski Medical Research Centre, Polish Academy of Sciences, 02-106 Warsaw, Poland

8Department of Cell Ultrastructure, Mossakowski Medical Research Centre, Polish Academy of Sciences, 02-106 Warsaw, Poland

9Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland

Corresponding author

Citation Information: Biological Chemistry. Volume 390, Issue 4, Pages 361–371, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2009.042, March 2009

Publication History

Received:
2008-11-21
Accepted:
2009-01-31
Published Online:
2009-03-05

Abstract

Circulating neutrophils and monocytes constitute the first line of antibacterial defence, which is responsible for the phagocytosis and killing of microorganisms. Previously, we have described that the staphylococcal cysteine proteinase staphopain B (SspB) cleaves CD11b on peripheral blood phagocytes, inducing the rapid development of features of atypical cell death in protease-treated cells. Here, we report that exposure of phagocytes to SspB critically impairs their antibacterial functions. Specifically, SspB blocks phagocytosis of Staphylococcus aureus by both neutrophils and monocytes, represses their chemotactic activity and induces extensive, nonphlogistic clearance of SspB-treated cells by macrophages. The proteinase also cleaves CD31, a major repulsion (‘do not-eat-me’) signal, on the surface of neutrophils. We suggest that both proteolytic degradation of repulsion signals and induction of ‘eat-me’ signals on the surface of leukocytes are responsible for the observed intensive phagocytosis of SspB-treated neutrophils by human monocyte-derived macrophages. Collectively, this may lead to the depletion of functional neutrophils at the site of infection, thus facilitating staphylococcal colonisation and spreading.

Keywords: clearance; engulfment; infection; inflammation; phagocytosis; protease; receptor; Staphylococcus aureus

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