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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


SCImago Journal Rank (SJR): 1.550
Source Normalized Impact per Paper (SNIP): 0.734

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Release of endo-lysosomal cathepsins B, D, and L from IEC6 cells in a cell culture model mimicking intestinal manipulation

Kristina Mayer1 / Anna Vreemann1 / Hong Qu1 / Klaudia Brix1

1School of Engineering and Science, Jacobs University Bremen, Campus Ring 6, D-28759 Bremen, Germany

Corresponding author

Citation Information: Biological Chemistry. Volume 390, Issue 5/6, Pages 471–480, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2009.047, March 2009

Publication History

Received:
2008-11-10
Accepted:
2009-02-16
Published Online:
2009-03-05

Abstract

IEC6 cells were used as an in vitro model system to study the effects of cell damage caused by mechanical manipulation of intestine epithelial cells. We constructed an apparatus that allowed analyzing the consequences of mechanical compression in a standardized and reproducible manner. Manipulation of IEC6 cells induced necrosis rather than apoptosis, and resulted in release of HMGB1, which is known to function as a trigger of inflammatory responses in vivo. Mechanical damage by traumatic injury of the intestine is accompanied by altered protease activities in the extracellular space, but only little is known about the possible contribution of endo-lysosomal cathepsins. Therefore, we tested the supernatants of manipulated cells in our in vitro model system for proteolytic activity and determined release rates by fluorimetric assays. Endo-lysosomal proteases, such as cathepsins B, D, and L, were released from damaged cells within the first 3 h after manipulation. While cathepsin L re-associated with the surfaces of neighboring cells, cathepsins B and D were present in the extracellular space as soluble enzymes. We conclude that our apparatus for mechanical manipulation can be used to approach surgical trauma, thereby focusing on epithelial cells of the intestine mucosa.

Keywords: aspartic proteases; cysteine proteases; intestine epithelial cell; mechanical compression; proteolysis; surgical trauma

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