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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred


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1437-4315
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Glycosylation pattern of brush border-associated glycoproteins in enterocyte-like cells: involvement of complex-type N-glycans in apical trafficking

Willy Morelle1 / Laurence Stechly2, 3 / Sabine André4 / Isabelle Van Seuningen2 / Nicole Porchet2, 3 / Hans-Joachim Gabius4 / Jean-Claude Michalski1 / Guillemette Huet2, 3

1UMR CNRS 8576, Unité de Glycobiologie Structurale et Fonctionnelle, IFR 147, F-59655 Villeneuve d'Ascq, France

2Centre de Recherche Jean-Pierre Aubert, INSERM U837, CHRU, F-59045 Lille cedex, France

3Centre de Biologie Pathologie Pierre Degand, CHRU, F-59037 Lille cedex, France

4Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximililans University Munich, D-80539 Munich, Germany

Corresponding author

Citation Information: Biological Chemistry. Volume 390, Issue 7, Pages 529–544, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2009.075, May 2009

Publication History

Received:
2009-02-11
Accepted:
2009-04-03
Published Online:
2009-05-09

Abstract

We have previously reported that galectin-4, a tandem repeat-type galectin, regulates the raft-dependent delivery of glycoproteins to the apical brush border membrane of enterocyte-like HT-29 cells. N-Acetyllactos-amine-containing glycans, known as galectin ligands, were found enriched in detergent-resistant membranes. Here, we analyzed the potential contribution of N- and/or O-glycans in this mechanism. Structural studies were carried out on the brush border membrane-enriched fraction using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and nano-ESI-QTOF-MS/MS. The pattern of N-glycans was very heterogeneous, with the presence of high mannose- and hybrid-type glycans as well as a multitude of complex-type glycans. In contrast, the pattern of O-glycans was very simple with the presence of two major core type 1 O-glycans, sialylated and bisialylated T-antigen structures [Neu5Acα2–3Galβ1–3GalNAc-ol and Neu5Acα2– 3Galβ1–3(Neu5Acα2–6)GalNAc-ol]. Thus, N-glycans rather than O-glycans contain the N-acetyllactosamine recognition signals for the lipid raft-based galectin-4-dependent apical delivery. In the presence of 1-deoxymannojirimycin, a drug which inhibits the generation of hybrid-type or complex type N-glycans, the extensively O-glycosylated mucin-like MUC1 glycoprotein was not delivered to the apical brush border but accumulated inside the cells. Altogether, our data demonstrate the crucial role of complex N-glycans in the galectin-4-dependent delivery of glycoproteins to the apical brush border membrane of enterocytic HT-29 cells.

Keywords: brush border; detergent-resistant membrane (DRM); galectin-4; glycosphingolipids; N-glycans; O-glycans

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