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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

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Angiotensin I-converting enzyme (ACE) activity and expression in rat central nervous system after sleep deprivation

Bruna Visniauskas1 / Vitor Oliveira2 / Adriana K. Carmona2 / Vânia D’Almeida1 / Robson L. de Melo3 / Sérgio Tufik1 / 1, 4

1Departamento de Psicobiologia, Universidade Federal de São Paulo (UNIFESP), Rua Napole;atao de Barros, 925, 04024-002, São Paulo, SP, Brazil

2Departamento de Biofísica, Universidade Federal de São Paulo (UNIFESP), Rua Pedro de Toledo, 669, 04039-032, São Paulo, SP, Brazil

3Center for Applied Toxinology (CAT/CEPID), Butantan Institute, Rua Vital Brasil, 1500, 05503-900, São Paulo, SP, Brazil

4Departamento de Ciências da Saúde, Universidade Federal de São Paulo (UNIFESP), Rua Ana Costa 95, 11060-001, São Paulo, SP, Brazil

Corresponding author

Citation Information: Biological Chemistry. Volume 392, Issue 6, Pages 547–553, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: 10.1515/bc.2011.057, June 2011

Publication History

Published Online:


Proteases are essential either for the release of neuropeptides from active or inactive proteins or for their inactivation. Neuropeptides have a fundamental role in sleep-wake cycle regulation and their actions are also likely to be regulated by proteolytic processing. Using fluorescence resonance energy transfer substrates, specific protease inhibitors and real-time PCR we demonstrate changes in angiotensin I-converting enzyme (ACE) expression and proteolytic activity in the central nervous system in an animal model of paradoxical sleep deprivation during 96 h (PSD). Male rats were distributed into five groups (PSD, 24 h, 48 h and 96 h of sleep recovery after PSD and control). ACE activity and mRNA levels were measured in hypothalamus, hippocampus, brainstem, cerebral cortex and striatum tissue extracts. In the hypothalamus, the significant decrease in activity and mRNA levels, after PSD, was only totally reversed after 96 h of sleep recovery. In the brainstem and hippocampus, although significant, changes in mRNA do not parallel changes in ACE specific activity. Changes in ACE activity could affect angiotensin II generation, angiotensin 1–7, bradykinin and opioid peptides metabolism. ACE expression and activity modifications are likely related to some of the physiological changes (cardiovascular, stress, cognition, metabolism function, water and energy balance) observed during and after sleep deprivation.

Keywords: ACE; angiotensin II; central nervous system; hypothalamus; paradoxical sleep deprivation; protease

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