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Trichobilharzia regenti: Antigenic structures of intravertebrate stages

1Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University in Prague, 128 00, Prague, Czech Republic

2Department of Parasitology, Faculty of Science, Charles University in Prague, 128 44, Prague, Czech Republic

3Department of Microbiology, 3rd Faculty of Medicine, Charles University in Prague, 100 34, Prague, Czech Republic

© 2011 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

Citation Information: Open Life Sciences. Volume 7, Issue 1, Pages 83–90, ISSN (Online) 2391-5412, DOI: 10.2478/s11535-011-0074-0, December 2011

Publication History

Published Online:
2011-12-25

Abstract

Like several other bird schistosomes, neurotropic schistosome of Trichobilharzia regenti can invade also mammals, including humans. Repeated infections cause cercarial dermatitis, a skin inflammatory reaction leading to parasite elimination in non-specific mammalian hosts. However, in experimentally primo-infected mice, the worms escape from the skin and migrate to the central nervous system. In order to evade host immune reactions, schistosomes undergo cercaria/schistosomulum transformation accompanied with changes of surface antigens. The present study is focused on localization of the main antigens of T. regenti; cercariae, schistosomula developed under different conditions and adults were compared. Antigens were localized by immunofluorescence and ultrastructural immunocytochemistry using sera of mice repeatedly infected with T. regenti. Detected antibody targets were located in glycocalyx and penetration glands of cercariae and in tegument of cercariae, schistosomula and adults. Shedding of cercarial glycocalyx significantly reduced surface reactivity; further decrease was reported during ongoing development of schistosomula. Spherical bodies, probably transported from subtegumental cell bodies to worm surface, were identified as the most reactive tegumental structures. Based on similar results for schistosomula developed in specific, non-specific hosts and in vitro, it seems that the ability of T. regenti to decrease the surface immunoreactivity during ontogenesis is independent on the host type.

Keywords: Trichobilharzia regenti; Neurotropic schistosome; Immunoreactivity; Immunolocalization; Ultrastructure

  • [1] Horák P., Dvořák J., Kolářová L., Trefil L., Trichobilharzia regenti, a pathogen of the avian and mammalian central nervous systems, Parasitology, 1999, 119, 577–581 http://dx.doi.org/10.1017/S0031182099005132

  • [2] Lichtenbergová L., Lassmann H., Jones M.K., Kolářová L., Horák P., Trichobilharzia regenti: Host immune response in the pathogenesis of neuroinfection in mice, Exp. Parasitol., 2011, 128, 328–335 http://dx.doi.org/10.1016/j.exppara.2011.04.006

  • [3] Hrádková K., Horák P., Neurotropic behaviour of Trichobilharzia regenti in ducks and mice, J. Helminthol., 2002, 76, 137–141 http://dx.doi.org/10.1079/JOH2002113

  • [4] Kouřilová P., Hogg K.G., Kolářová L., Mountford A.P., Cercarial dermatitis caused by bird schistosomes comprises both immediate and late phase cutaneous hypersensitivity reactions, J. Immunol., 2004, 172, 3766–3774

  • [5] Lichtenbergová L., Kolbeková P., Kouřilová P., Kašný M., Mikeš L., Haas H., et al., Antibody responses induced by Trichobilharzia regenti antigens in murine and human hosts exhibiting cercarial dermatitis, Parasite Immunol., 2008, 30, 585–595

  • [6] Kouřilová P., Kolářová L., Variations in immunofluorescent antibody response against Trichobilharzia and Schistosoma antigens in compatible and incompatible hosts, Parasitol. Res., 2002, 88, 513–521 http://dx.doi.org/10.1007/s00436-002-0607-6

  • [7] Hockley D.J., McLaren D.J., Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercaria to adult worm, Int. J. Parasitol., 1973, 3, 13–25 http://dx.doi.org/10.1016/0020-7519(73)90004-0

  • [8] Horák P., Kovář L., Kolářová L., Nebesářová J., Cercaria-schistosomulum surface transformation of Trichobilharzia szidati and its putative immunological impact, Parasitology, 1998, 116, 139–147 http://dx.doi.org/10.1017/S0031182097002059

  • [9] El Ridi R., Mohamed S.H., Tallima H., Incubation of Schistosoma mansoni lung-stage schistosomula in corn oil exposes their surface membrane antigenic specificities, J. Parasitol., 2003, 89, 1064–1067 http://dx.doi.org/10.1645/GE-3122RN

  • [10] Lawson B.W., Bickle Q.D., Taylor M.G., Mechanisms involved in the loss of antibodymediated adherence of macrophages to lungstage schistosomula of Schistosoma mansoni in vitro, Parasitology, 1993, 106, 463–469 http://dx.doi.org/10.1017/S0031182000076757

  • [11] Parizade M., Arnon R., Lachmann P.J., Fishelson Z., Functional and antigenic similarities between a 94-kDa protein of Schistosoma mansoni (SCIP-1) and human CD59, J. Exp. Med., 1994, 179, 1625–1636 http://dx.doi.org/10.1084/jem.179.5.1625

  • [12] Abath F.G.C., Werkhauser R.C., The tegument of Schistosoma mansoni: functional and immunological features, Parasite Immunol., 1996, 18, 15–20 http://dx.doi.org/10.1046/j.1365-3024.1996.d01-6.x

  • [13] Skelly P.J., Wilson A.R., Making sense of the schistosome surface, Adv. Parasit., 2006, 63, 185–284 http://dx.doi.org/10.1016/S0065-308X(06)63003-0

  • [14] Loukas A., Tran M., Pearson M.S., Schistosome membrane proteins as vaccines, Int. J. Parasitol., 2007, 37, 257–263 http://dx.doi.org/10.1016/j.ijpara.2006.12.001

  • [15] Chanová M., Bulantová J., Máslo P., Horák P., In vitro cultivation of early schistosomula of nasal and visceral bird schistosomes (Trichobilharzia spp., Schistosomatidae), Parasitol. Res., 2009, 104, 1445–1452 http://dx.doi.org/10.1007/s00436-009-1343-y

  • [16] Horák P., Kolářová L., Dvořák J., Trichobilharzia regenti n. sp. (Schistosomatidae, Bilharziellinae) a new nasal schistosome from Europe, Parasite, 1998, 5, 349–357

  • [17] Marikovsky M., Arnon R., Fishelson Z., Proteases secreted by transforming schistosomula of Schistosoma mansoni promote resistance to killing by complement, J. Immunol., 1988, 141, 273–278

  • [18] McKerrow J.H., Jones P., Sage H., Pino-Heiss S., Proteinases from invasive larvae of the trematode parasite Schistosoma mansoni degrade connective-tissue and basement-membrane macromolecules, Biochem. J., 1985, 231, 47–51

  • [19] Fishelson Z., Amiri P., Friend D.S., Marikovsky M., Petitt M., Newport G., et al., Schistosoma mansoni: cell-specific expression and secretion of a serine protease during development of cercariae, Exp. Parasitol., 1992, 75, 87–98 http://dx.doi.org/10.1016/0014-4894(92)90124-S

  • [20] Ligasová A., Bulantová J., Šebesta O., Kašný M., Koberna K., Mikeš L., Secretory glands in cercariae of the neuropathogenic schistosome Trichobilharzia regenti — ultrastructural characterization, 3-D modeling, volume and pH estimations, Parasit Vectors., 2011, 4, 162 http://dx.doi.org/10.1186/1756-3305-4-162

  • [21] Dorsey C.H., Cousin C.E., Lewis F.A., Stirewalt M.A., Ultrastructure of the Schistosoma mansoni cercariae, Micron, 2002, 33, 279–323 http://dx.doi.org/10.1016/S0968-4328(01)00019-1

  • [22] Wilson R.A., Barnes P.E., The tegument of Schistosoma mansoni: observations on the formation, structure and composition of cytoplasmic inclusions in relation to tegument function, Parasitology, 1974, 68(2), 239–258

  • [23] Gobert G.N., Stenzel D.J., McManus D.P., Jones M.K., The ultrastructural architecture of the adult Schistosoma japonicum tegument, Int. J. Parasitol., 2003, 33, 1561–1575 http://dx.doi.org/10.1016/S0020-7519(03)00255-8

  • [24] Payares G., McLaren D.J., Evans W.H., Smithers S.R., Changes in the surface antigen profile of Schistosoma mansoni during maturation from cercaria to adult worm, Parasitology, 1985, 91, 83–99 http://dx.doi.org/10.1017/S0031182000056535

  • [25] Pearce E.J., Basch P.F., Sher A., Evidence that the reduced surface antigenicity of developing Schistosoma mansoni schistosomula is due to antigen shedding rather than host molecule acquisition, Parasite Immunol., 1986, 8, 79–94 http://dx.doi.org/10.1111/j.1365-3024.1986.tb00835.x

  • [26] McManus D.P., Loukas A., Current status of vaccines for Schistosomiasis, Clin. Microbiol. Rev., 2008, 21, 225–242 http://dx.doi.org/10.1128/CMR.00046-07

  • [27] Kašný M., Lichtenbergová L., Kolářová L., Horák P., Identification and characterization of dominant antigens of the bird schistosome Trichobilharzia regenti, In: L. Mikeš, P. Horák (Eds.), Abstract Book — 3rd Workshop on Bird Schistosomes and Cercarial Dermatitis (6–10 July 2009, Rejčkov, Ledeč nad Sázavou, Czech Republic), Charles University in Prague, Faculty of Science, 2009, 21

  • [28] Goudot-Crozel V., Caillol D., Djabali M., Dessein A.J., The major parasite surface antigen associated with human resistance to schistosomiasis is a 37-kD glyceraldehyde-3 P-dehydrogenase, J. Exp. Med., 1989, 170, 2065–2080 http://dx.doi.org/10.1084/jem.170.6.2065

  • [29] Henkle K.J., Davern K.M., Wright M.D., Ramos A.J., Mitchell G.F., Comparison of the cloned genes of the 26- and 28- kilodalton S-transferases of Schistosoma japonicum and Schistosoma mansoni, Mol. Biochem. Parasitol., 1990, 40, 23–34 http://dx.doi.org/10.1016/0166-6851(90)90076-X

  • [30] Matsumoto Y., Perry G., Levine R.J., Blanton R., Mahmoud A.A., Aikawa M., Paramyosin and actin in schistosomal teguments, Nature, 1988, 5, 76–78 http://dx.doi.org/10.1038/333076a0

  • [31] Gobert G.N., Stenzel D.J., Jones M.K., Allen D.E., McManus D.P., Schistosoma japonicum: immunolocalization of paramyosin during development, Parasitology, 1997, 114, 45–52 http://dx.doi.org/10.1017/S0031182096008001

  • [32] Nara T., Matsumoto N., Janecharut T., Matsuda H., Yamamoto K., Irimura T., et al., Demonstration of the target molecule of a protective IgE antibody in secretory glands of Schistosoma japonicum larvae, Int. Immunol., 1994, 6, 963–971 http://dx.doi.org/10.1093/intimm/6.7.963

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