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Biological Letters

The Journal of Adam Mickiewicz University, Faculty of Biology; Poznan Society for the Advancement of the Arts and Sciences

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Evaluation of the applicability of polymerase chain reaction (PCR) to sex identification in Eurasian blackbirds (Turdus merula)

Andrzej Dybus1 / Anna Siemierz1 / Dariusz Wysocki1 / Iwona Szatkowska1 / Magdalena Muszyńska1 / Sebastian Guenzel1

Laboratory of Molecular Cytogenetics, West Pomeranian University of Technology, Szczecin, Doktora Judyma 10, 71-460 Szczecin, Poland1

Department of Anatomy and Vertebrate Zoology, University of Szczecin, Wąska, 71-412 Szczecin, Poland2

Department of Poultry and Ornamental Bird Breeding, West Pomeranian University of Technology, Szczecin, Doktora Judyma 24, 71-466 Szczecin, Poland3

This content is open access.

Citation Information: Biological Letters. Volume 46, Issue 1, Pages 15–20, ISSN (Online) 1734-7467, ISSN (Print) 1644-7700, DOI: 10.2478/v10120-009-0009-x, July 2010

Publication History

Published Online:

Evaluation of the applicability of polymerase chain reaction (PCR) to sex identification in Eurasian blackbirds (Turdus merula)

Turdus merula is one of most common bird species in Europe. This study verified a method for its sex identification by PCR with P2/P8 primers, based on the CHD1 gene polymorphism, proposed in earlier studies as a universal tool for sex identification in most bird species. Although there are some reports that PCR cannot determine sex in Eurasian blackbirds due to a lack of differences in intron lengths of CHD1-Z and CHD1-W genes, our study showed that such a possibility does exist, so it can contribute to an increased understanding of the biology of this species.

Keywords: CHD1; PCR; molecular sexing; blackbird; Turdus merula

  • Bello N., Francino O., Sanchez A. 2001. Isolation of genomic DNA from feathers. J. Vet. Diagn. Invest. 13: 162-164.

  • Cheng Y-H., Kuo T-F., Lee D-N., Weng C-F. 2006. Sex Identification of the Black-faced Spoonbill (Platalea minor). Zool. Stud. 45: 104-113.

  • Chromas, v. 2.31. http://www.technelysium.com.au/chromas.html

  • Cramp S., Simmons K. E. K. (eds). 1988. The birds of the Western Palearctic. Oxford Univ. Press.

  • Dawson D. A., Darby S., Hunter F. M., Krupa A. P., Jones I. L., Burke T. 2001. A critique of avian CHD-based molecular sexing protocols illustrated by a Z-chromosome polymorphism detected in auklets. Mol. Ecol. Notes. 1: 201-204.

  • Eason D., Millar C. D., Cree A., Halverson J., Lambert D. M. 2001. A comparison of five methods for assignment of sex in the takahe (Aves: Porphyrio mantelli). J. Zool. 253: 281-292.

  • Ellegren H. 1996. First gene on the avian W chromosome (CHD) provides a tag for universal sexing of non-ratite birds. Proc. Biol. Sci. 263: 1635-1644.

  • Ellegren H., Carmichael A. 2001. Multiple and independent cessation of recombination between avian sex chromosomes. Genetics. 158: 325-331.

  • Ellegren H., Sheldon B. C. 1997. New tools for sex identification and the study of sex allocation in birds. Trends Ecol. Evol. (Amst.). 12: 255-259.

  • Ewen G. E., Cassey P., Mřller A. P. 2004. Facultative primary sex ratio variation: a lack of evidence in birds? Proc. Biol. Sci. 171: 1277-1283.

  • Fridolfsson A. K., Ellegren H. 1999. A simple and universal method for molecular sexing of nonratite birds. J. Avian Biol. 30: 116-121.

  • Griffiths R., Double M., Orr K., Dawson R. 1998. A DNA test to sex most birds. Mol. Ecol. 7: 1071-1075.

  • Griffiths R., Korn R. 1997. A CHD1 gene is Z chromosome linked in the chicken Gallus domesticus. Gene. 197: 225-229.

  • Griffiths R., Tiwari B. 1995. Sex of the last wild Spix's macaw. Nature. 375: 454.

  • Ito H., Sudo-Yamaji A., Abe M., Murase T., Tsubota T. 2003. Sex identification by alternative polymerase chain reaction methods in falconiformes. Zool. Sci. 20: 339-344.

  • Magrath R. D. 1989. Hatching asynchrony and reproductive success in the Blackbird. Nature. 339: 536-538.

  • Magrath R. D. 1991. Nestling weight and juvenile survival in the Blackbird, Turdus merula. J. Anim. Ecol. 60: 335-351.

  • Mulsow R., Tomialojć L. 1997. Blackbird. In: The EBCC Atlas of European Breeding Birds: Their Distribution and Abundance (Hagemeijer WJM, Blair MJ, eds.), T & A D Poyser, London. 544-545.

  • Nesje M., Rřed K. H. 2000. Sex identification in falcons using microsatellite DNA markers. Hereditas. 132: 261-263.

  • Newton I. 2004. The recent declines of farmland bird populations in Britain: an appraisal of causal factors and conservation actions. Ibis. 146: 579-600.

  • Sacharczuk M., Jaszczak K., Kruszewicz A. 2002. Sex identification in monomorphic birds - a review. Pr. Mater. Zoot. 60: 35-50.

  • Szatkowska I., Zych S., Kulig H. 2003. Sex determination in mammals. Med. Weter. 59, 847-852.

  • Wysocki D. 2004. Within-season divorce rate in an urban population of European Blackbird Turdus merula. Ardea. 92: 219-228.

  • Wysocki D. 2006. Factors affecting the between-season divorce rate in the urban population of European Blackbird Turdus merula in north-western Poland. Acta Ornithol. 41: 71-78.

  • Zhang Z., Schwartz S., Wagner L., Miller W. 2000. A greedy algorithm for aligning DNA sequences. J. Comput. Biol. 7:203-14.

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