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Publication Date:
16 11 2011
ISSN:
1437-4323
DOI:
10.1515/BOT.2011.067

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Editor-in-Chief: Chapman, Anthony R. O.

null Enriquez Dominguez, Susana / Heimann, Kirsten / Pohnert, Georg / Poulin, Michel / Shearer, Carol A. / Amsler, Charles D. / Beardall, John / Berges, John A. / Campbell, Jinx / Dawes, Clinton J. / Gomez, Ivan / Hoppenrath, Mona / Wynne, Michael J. / Yoon, Hwan Su

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Identification of hypo-osmotically induced genes in Kappaphycus alvarezii (Solieriaceae, Rhodophyta) through expressed sequence tag analysis

1 / Wang, Xiuliang 2 / Huang, Xiaohang 1 / Liu, Jianguo 2

1The First Institute of Oceanography, Marine Ecological Center, State Oceanic Administration, Qingdao 266061, China

2Institute of Oceanology, The Chinese Academy of Sciences, Qingdao 266071, China

Corresponding author

Citation Information: Botanica Marina. Volume 54, Issue 6, Pages 557–562, ISSN (Online) 1437-4323, ISSN (Print) 0006-8055, DOI: 10.1515/BOT.2011.067, November 2011

Publication History:

Received: 06/07/2011;
Accepted: 13/10/2011;
Published Online: 26/02/2012

Abstract

Hypo-osmotic conditions are among the most stressful environmental factors that affect seaweed physiology and productivity. To improve understanding of hypo-osmotic adaptation and to find genes involved in low salinity stress, we conducted an analysis of expressed sequence tags (ESTs) on the κ-carrageenan-producing red algae, Kappaphycus alvarezii. Of 454 tentatively unique genes (TUGs), 281 matched protein sequences by BlastX analysis (e value≤10-3), 112 TUGs matched ESTs from other agar- or carrageenan-producing red algae, and 98 TUGs were annotated. The transcriptional profiles of six functional genes from K. alvarezii ESTs were examined. An ATP-binding cassette transporter gene and a sodium phosphate symporter gene were significantly up-regulated, indicating the importance of membrane transport proteins in coping with hypo-osmotic stress conditions.

Keywords: ATP-binding cassette transporter; expressed sequence tag; hypo-osmotic stress; Kappaphycus alvarezii; sodium phosphate symporter

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