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Publication Date:
June 2005
ISSN:
1437-4331
DOI:
10.1515/CCLM.1998.060

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Clinical Chemistry and Laboratory Medicine (CCLM)

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Engineering of an Anti-Steroid Antibody: Amino Acid Substitutions Change Antibody Fine Specificity from Cortisol Estradiol

Patrick Chames / Daniel Baty

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 36, Issue 6, Pages 355–359, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.1998.060, June 2005

Publication History:
Published Online:
2005-06-01

Abstract

Immunoassays are widely used for determination of the concentration of steroid hormones. However, obtaining specific anti-steroid monoclonal antibodies remains difficult. We used antibody engineering and phage display methods to change the specificity of an anti-cortisol monoclonal antibody towards estradiol. This work demonstrates that production of recombinant antibodies may be a valuable way of obtaining the high-specificity antibodies required for steroid immunoassays.

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