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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Editorial Board Member: Gillery, Philippe / Kazmierczak, Steven / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Whitfield, John B.

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Determination of Ascorbic Acid in Plasma and Urine by High Performance Liquid Chromatography with Ultraviolet Detection

Andreas Rümelin / Ulrich Fauth / Miklos Halmágyi

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 37, Issue 5, Pages 533–536, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.1999.086, June 2005

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A reliable simple reversed-phase liquid chromatographic method for the routine determination of ascorbic acid in plasma and urine with ultraviolet detection is described. This method enables the complete separation of the ascorbic acid peak from others with a recovery of above 95 % within 8 minutes. The method can be used for analysing multiple samples within a day. In addition, the storage conditions and stability of ascorbic acid in plasma and urine were investigated. Samples of plasma and urine can be stored on ice in darkness for at least 60 min without reduction of ascorbic acid concentration. Prepared samples can be stored in darkness at 4 °C for at least 120 min and in liquid nitrogen for 42 days.

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