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Publication Date:
June 2005
ISSN:
1437-4331
DOI:
10.1515/CCLM.2000.025

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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the International Federation of Clinical Chemistry and Laboratory Medicine and the European Federation of Clinical Chemistry and Laboratory Medicine

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Unravelling Genetic Data by Arrayed Primer Extension

Neeme Tõnisson / Ants Kurg / Krista Kaasik / Elin Lõhmussaar / Andres Metspalu

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 38, Issue 2, Pages 165–170, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2000.025, June 2005

Publication History:
Published Online:
2005-06-01

Abstract

We have developed a method for arrayed primer extension (APEX) on an oligonucleotide microchip together with the 4-color fluoresence imaging equipment and supporting software, that allows analysis of the DNA sequence and changes in it. Mutation analysis of BRCA1 gene and single nucleotide polymorphism (SNP) chip for genotyping were used as a model system. Chip surface chemistry, template preparation and APEX reaction conditions were optimised and the assay is ready to be implemented in variety of DNA analysis from SNP testing to DNA resequencing.

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