Evaluation of a New Simple and Rapid Enzyme-Linked Immunosorbent Assay Kit for Neopterin Determination : Clinical Chemistry and Laboratory Medicine Jump to ContentJump to Main Navigation
Show Summary Details

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R.


IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238

249,00 € / $374.00 / £187.00*

Online
ISSN
1437-4331
See all formats and pricing

 


Select Volume and Issue
Loading journal volume and issue information...

Evaluation of a New Simple and Rapid Enzyme-Linked Immunosorbent Assay Kit for Neopterin Determination

Jürgen Westermann / Frank Thiemann / Ludwig Gerstner / Franz Tatzber / Ivan Kozák / Thomas Bertsch / Christian Krüger

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 38, Issue 4, Pages 345–353, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2000.050, June 2005

Publication History

Published Online:
2005-06-01

Abstract

A new commercially available enzyme-linked immunosorbent assay (ELISA) kit has been evaluated for the measurement of neopterin concentrations in serum, plasma and urine. This competitive ELISA is technically simple, requires only small sample volume and is rapid to perform. The assay procedure consists of sequential 1.5 h and 10 min room temperature incubation steps. The ELISA is accurate, sensitive, specific, and precise. Linear regression analysis of neopterin concentrations measured with the new ELISA and with an established method yielded a highly singificant correlation (r = 0.99). The new assay is applicable to ELISA workstations, thus enabling determination of neopterin in large series of samples. The neopterin ELISA kit has been used in routine laboratory testing of blood donations in a blood bank.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Sonia Centi, Sara Tombelli, Mariarita Puntoni, Claudio Domenici, Milan Franek, and Ilaria Palchetti
Talanta, 2015, Volume 134, Page 48
[2]
Liliana Elena D'agostino, Fernando Ventimiglia, Juan Antonio Verna, Amelia De La Colina, Yanina Aguirre, Alfredo Arturi, and Alberto Capparelli
Autoimmunity, 2013, Volume 46, Number 1, Page 44
[3]
Nevbahar Turgan, Sara Habif, Zuhal Parildar, Dilek Özmen, Isil Mutaf, Dilek Erdener, and Oya Bayindir
Clinical Biochemistry, 2001, Volume 34, Number 4, Page 271
[5]
Ursula Sauer, Patrick Domnanich, and Claudia Preininger
Analytical Biochemistry, 2011, Volume 419, Number 1, Page 46
[6]
A Laich, G Neurauter, B Wirleitner, and D Fuchs
Clinica Chimica Acta, 2002, Volume 322, Number 1-2, Page 175
[7]
Elizabeth A. Flavall, Elizabeth M. Crone, Grant A. Moore, and Steven P. Gieseg
Journal of Chromatography B, 2008, Volume 863, Number 1, Page 167
[8]
Ivo Cernoch, Erwin Schleicher, and Milan Franek
Analytical and Bioanalytical Chemistry, 2011, Volume 399, Number 2, Page 979
[9]
E. A. Silva, A. Iyer, S Ura, J. R. Lauris, B. Naafs, P. K. Das, and F. Vilani-Moreno
Tropical Medicine & International Health, 2007, Volume 12, Number 12, Page 1450
[10]
S P Gieseg, E M Crone, E A Flavall, and Z Amit
British Journal of Pharmacology, 2008, Volume 153, Number 4, Page 627
[11]
Malte Ziemann, Sabine Krueger, Andrea B. Maier, Alexander Unmack, Siegfried Goerg, and Holger Hennig
Transfusion, 2007, Volume 47, Number 11, Page 1972
[12]
C. Micha Nübling, Michael Chudy, Peter Volkers, and Johannes Löwer
Transfusion, 2006, Volume 46, Number 11, Page 1886

Comments (0)

Please log in or register to comment.