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Publication Date:
June 2005
ISSN:
1437-4331
DOI:
10.1515/CCLM.2000.050

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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the International Federation of Clinical Chemistry and Laboratory Medicine and the European Federation of Clinical Chemistry and Laboratory Medicine

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Evaluation of a New Simple and Rapid Enzyme-Linked Immunosorbent Assay Kit for Neopterin Determination

Jürgen Westermann / Frank Thiemann / Ludwig Gerstner / Franz Tatzber / Ivan Kozák / Thomas Bertsch / Christian Krüger

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 38, Issue 4, Pages 345–353, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2000.050, June 2005

Publication History:
Published Online:
2005-06-01

Abstract

A new commercially available enzyme-linked immunosorbent assay (ELISA) kit has been evaluated for the measurement of neopterin concentrations in serum, plasma and urine. This competitive ELISA is technically simple, requires only small sample volume and is rapid to perform. The assay procedure consists of sequential 1.5 h and 10 min room temperature incubation steps. The ELISA is accurate, sensitive, specific, and precise. Linear regression analysis of neopterin concentrations measured with the new ELISA and with an established method yielded a highly singificant correlation (r = 0.99). The new assay is applicable to ELISA workstations, thus enabling determination of neopterin in large series of samples. The neopterin ELISA kit has been used in routine laboratory testing of blood donations in a blood bank.

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