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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R. / Tsongalis, Gregory J.

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The White Blood Cell Differential: Three Methods Compared

Rüdiger Siekmeier / Alexa Bierlich / Werner Jaroß

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 39, Issue 5, Pages 432–445, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2001.069, June 2005

Publication History

Published Online:
2005-06-01

Abstract

The analysis of the automated blood cell count is an essential tool in haematological diagnostics. However, in the case of the white blood cell differential the microscopy method, although tedious, often serves as reference. We evaluated the ABX Pentra 120 Retic haematology analyser in comparison to the Coulter STKS haematology system and the microscopy method with respect to accuracy, precision and reliability. We compared 308 samples (239 samples from adults and 69 from children) including patients with oncological diseases. The comparison of the white blood cell differential revealed strong correlations between the results obtained with the ABX Pentra 120 Retic and the microscopy method, the Coulter STKS and the microscopy method and both automated methods (values of paediatric samples in parentheses; neutrophils: rS≥0.933 (rS≥0.951), lymphocytes: rS≥0.907 (rS≥0.945), monocytes: rS≥0.584 (rS≥0.459) and eosinophils: rS≥0.963 (rS≥0.966)). The analytical performance of automatic analysers for the detection of the morphological “left shift” was determined for all samples in comparison to the microscopical white blood cell differential. The sensitivity, specificity and efficiency depended strongly on the chosen threshold levels and were different for both analysers. The sensitivity for flagging a left shift increased with an increasing proportion of neutrophil bands, metamyelocytes, myelocytes and promyelocytes. Our study suggests that the ABX Pentra 120 Retic haematology analyser, as well as the Coulter STKS haematology system are useful tools for routine analysis in haematology.

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[1]
N. Nomura, K. Saito, M. Ikeda, S. Yuasa, M. Pastore, C. Chabert, E. Kono, A. Sakai, H. Tanaka, T. Ikemoto, and T. Takubo
International Journal of Laboratory Hematology, 2014, Page n/a
[2]
Ah Hyun Kim, Wonbae Lee, Myungshin Kim, Yonggoo Kim, and Kyungja Han
Blood Research, 2014, Volume 49, Number 2, Page 120
[3]
Jan Van den Bossche, Katrien Devreese, Ronald Malfait, Martine Van de Vyvere, Annick Wauters, Hugo Neels, and Pieter De Schouwer
Clinical Chemistry and Laboratory Medicine, 2002, Volume 40, Number 1
[4]
K. GUERTI, F. VERTESSEN, L. DANIËLS, and M. VAN DER PLANKEN
International Journal of Laboratory Hematology, 2009, Volume 31, Number 2, Page 132
[5]
Kay L. Lantis, MT(ASCP) SH, R. Jayne Harris, MT(ASCP), Gerald Davis, MPH MT(ASCP), Nancy Renner, MT(ASCP) SH, and William G. Finn, MD
American Journal of Clinical Pathology, 2003, Volume 119, Number 5, Page 656

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