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Publication Date:
June 2005
ISSN:
1437-4331
DOI:
10.1515/CCLM.2001.086

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Clinical Chemistry and Laboratory Medicine (CCLM)

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Sensitive Chemiluminescence Immunoassay for the Determination of Rat Serum α1-Acid Glycoprotein

Jochen Metzger / Manfred Blobner / Peter B. Luppa

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 39, Issue 6, Pages 514–518, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2001.086, June 2005

Publication History:
Published Online:
2005-06-01

Abstract

We present the establishment of a sensitive immunoassay for the determination of α1-acid glycoprotein (orosomucoid, AGP) in rat serum. The assay is based upon antigen capture by surface-immobilized specific polyclonal rabbit anti-AGP antibodies with biotinylated rat AGP (rAGP) as the tracer, and formatted as competitive enzyme immunoassay. Signaling is performed by streptavidin-conjugated horseradish peroxidase. Enzyme activity is quantified by an enhanced chemiluminometric method, allowing the sensitive detection of rAGP serum levels in small sample volumes.

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