Clinical Chemistry and Laboratory Medicine (CCLM)
Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)
Editor-in-Chief: Plebani, Mario
Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R.
IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition
SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238
Determination of Nitrite Plus Nitrate and Malondialdehyde in Human Plasma: Analytical Performance and the Effect of Smoking and Exercise
Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 40, Issue 8, Pages 802–809, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2002.139, June 2005
- Published Online:
The aim of this study was to evaluate the analytical performance and clinical usefulness of spectrophotometric assays for the measurement of the plasma levels of nitrite plus nitrate (NOx), and malondialdehyde (MDA), as an index of nitric oxide release and lipid peroxidation, respectively. We studied 30 healthy sedentary volunteers, 12 endurance athletes and 12 regular heavy smokers. The lower limit of quantification for plasma NOx concentration was 1 μmol/l, and linearity was observed from 1 to 40 μmol/l of NOx concentration. Variation in replicate samples within or between days was always below 5%. NOx levels were significantly higher in athletes compared to both control subjects and smokers (p<0.05 and p<0.001, respectively), as well as in healthy subjects compared to smokers (p<0.05). The analytical limit of quantification for plasma MDA concentration was 0.03 μmol/l, and linearity was observed from 0.03 to 20 μmol/l of MDA concentration. Variation in replicate samples within or between runs was <5%. Mean MDA concentration was significantly higher in smokers compared to control subjects and athletes (p<0.001). A significant inverse relationship (p<0.001) was observed when comparing NOx with MDA (r= −0.49) or LDL levels (r= −0.30) in the total population. The assays evaluated in this study proved to be sensitive, specific and practicable, and therefore suitable for routine application in clinical chemistry laboratories and/or physiopathological studies involving human blood
Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.