Jump to ContentJump to Main Navigation

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R. / Tsongalis, Gregory J.

12 Issues per year


IMPACT FACTOR 2014: 2.707
Rank 6 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2014: 0.741
Source Normalized Impact per Paper (SNIP) 2014: 1.011
Impact per Publication (IPP) 2014: 2.310

VolumeIssuePage

Issues

Screening of antinuclear antibodies: comparison between enzyme immunoassay based on nuclear homogenates, purified or recombinant antigens and immunofluorescence assay

Sergio Bernardini1 / Maria Infantino2 / Lorenza Bellincampi3 / Marzia Nuccetelli4 / Antonella Afeltra5 / Roberta Iori6 / Antonino Biroccio7 / Andrea Urbani8 / Giorgio Federici9

1.

2.

3.

4.

5.

6.

7.

8.

9.

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 42, Issue 10, Pages 1155–1160, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2004.235, October 2004

Publication History

Received:
July 9, 2004
Accepted:
July 12, 2004

Abstract

Current clinical practice considers antinuclear antibody (ANA) testing as a screening test; this has a major impact on laboratory work with a growing volume of analyses that need to be performed rapidly, to maintain good specificity and sensitivity. Ongoing discussions have been raised in order to identify the best technology to use in ANA screening, taking into account both clinical and economical implications. The aim of our study was to compare three different enzyme immunoassays (EIA) with immunofluorescence (IF) assay in order to identify which test is better for use as a screening test. The study was performed on 473 sera and the three different EIA tests were based on nuclear homogenates from HeLa cells, purified antigens from HEp-2 cells and recombinant antigens, respectively. The concordance between EIA-ANA and IF-ANA techniques, determined by the κ statistic, was acceptable, but not complete, and discrepancies between both EIA-positive/IF-negative samples and IF-positive/EIA-negative were found. Both methods show interesting diagnostic abilities, however, the IF-ANA assay seems to be the first choice test in a well-standardized immunofluorescence laboratory with experienced microscopists, whereas the EIA test might be useful especially in large-scale ANA screening.

Keywords: antinuclear antibody; immunofluorescence; enzyme immunoassay

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Michael Mahler, Pier-Luigi Meroni, Xavier Bossuyt, and Marvin J. Fritzler
Journal of Immunology Research, 2014, Volume 2014, Page 1
[2]
Aryeh M. Abeles and Micha Abeles
The American Journal of Medicine, 2013, Volume 126, Number 4, Page 342
[3]
Renato Tozzoli, Antonio Antico, Brunetta Porcelli, and Danila Bassetti
Autoimmunity Highlights, 2012, Volume 3, Number 2, Page 59
[4]
Marin Yordanov Zagorov, Marian Marinov Draganov, Stanislava Aleksandrova Alimanska, Nedyalka Doncheva Staykova, Rumen Stefanov Stefanov, Monica Trayancheva, Stefka Ivanova Kuzmanova, and Marianna Atanasova Murdjeva
Comparative Immunology, Microbiology and Infectious Diseases, 2007, Volume 30, Number 3, Page 153
[5]
Joseph P. Michalski and Charles Kodner
Primary Care: Clinics in Office Practice, 2010, Volume 37, Number 4, Page 767
[6]
Marilina Tampoia, Valentina Mastrandrea, Nicoletta Cassano, and Gino Antonio Vena
Immunopharmacology and Immunotoxicology, 2009, Volume 31, Number 1, Page 127
[7]
Marjan Van Blerk, Christel Van Campenhout, Xavier Bossuyt, Jean Duchateau, René Humbel, Geneviève Servais, Jean-Paul Tomasi, Adelin Albert, Wim Coucke, and Jean-Claude Libeer
Clinical Chemistry and Laboratory Medicine, 2009, Volume 47, Number 1
[8]
Ehud Paz, Muhammed Adawi, Idit Lavi, Yehuda Mussel, and Reuven Mader
Rheumatology International, 2007, Volume 27, Number 10, Page 941

Comments (0)

Please log in or register to comment.