Jump to ContentJump to Main Navigation

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R. / Tsongalis, Gregory J.

12 Issues per year

IMPACT FACTOR 2014: 2.707
Rank 6 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2014: 0.741
Source Normalized Impact per Paper (SNIP) 2014: 1.011
Impact per Publication (IPP) 2014: 2.310



Analysis of candidate genes in Polish families with obesity

Malgorzata Malczewska-Malec / Iwona Wybranska / Iwona Leszczynska-Golabek / Lukasz Partyka / Jadwiga Hartwich / Agata Jabrocka / Beata Kiec-Wilk / Malgorzata Kwasniak / Marcin Motyka / Aldona Dembinska-Kiec

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 42, Issue 5, Pages 487–493, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2004.083, June 2005

Publication History

Published Online:


This study analyzes the relationship between risk factors related to overweight/obesity, insulin resistance, lipid tolerance, hypertension, endothelial function and genetic polymorphisms associated with: i) appetite regulation (leptin, melanocortin-3-receptor (MCR-3), dopamine receptor 2 (D2R)); ii) adipocyte differentiation and insulin sensitivity (peroxisome proliferator-activated receptor-γ2 (PPAR-γ2), tumor necrosis factor-α (TNF-α)); iii) thermogenesis and free fatty acid (FFA) transport/catabolism (uncoupling protein-1 (UCP1), lipoprotein lipase (LPL), β2- and β3-adrenergic receptor (β2AR, β3AR), fatty acid transport protein-1 (FATP-1) and iv) lipoproteins (apoliprotein E (apoE), apo CIII).

The 122 members of 40 obese Caucasian families from southern Poland participated in the study. The genotypes were analyzed by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) or by direct sequencing. Phenotypes related to obesity (body mass index (BMI), fat/lean body mass composition, waist-to-hip ratio (WHR)), fasting lipids, glucose, leptin and insulin, as well as insulin during oral glucose tolerance test (OGTT) (4 points within 2 hours) and during oral lipid tolerance test (OLTT) (5 points within 8 hours) were assessed. The insulin sensitivity indexes: homeostasis model assessment of insulin resistance, whole body insulin sensitivity index, hepatic insulin sensitivity and early secretory response to an oral glucose load (HOMA-IR, ISI-COMP, ISI-HOMA and DELTA) were calculated.

The single gene mutations such as C105 T OB and Pro115 Gln PPAR-γ2 linked to morbid obesity were not detected in our group. A weak correlation between obesity and certain gene polymorphisms was observed. Being overweight (25<BMI≤30 kg/m2) significantly correlated with worse FFA tolerance in male PPAR-γ2 12Pro, LPL-H (G) allele carriers. Insulin resistance was found in female PPAR-γ2 Pro12, TNF-α (−308A) and LPL-H (G) allele carriers. Hypertension linked to the PPAR-γ2 Pro allele carriers was characterized by high leptin output during OLTT.

We conclude that the polymorphisms we investigated were weakly correlated with obesity but significantly modified the risk factors of the metabolic syndrome.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Eliane Lopes Rosado, Josefina Bressan, Marta F. Martins, Paulo R. Cecon, and Jose Alfredo Martínez
Appetite, 2007, Volume 49, Number 3, Page 635
M. Zawodniak-Szalapska, R. Stawerska, E. Brzeziańska, D. Pastuszak-Lewandoska, J. Lukamowicz, K. Cypryk, and A. Lewinski
Journal of Pediatric Endocrinology and Metabolism, 2008, Volume 21, Number 2
Yong-Jian Zhou, Yu-Yuan Li, Yu-Qiang Nie, Hui Yang, Qi Zhan, Jian Huang, Sheng-Li Shi, Xiao-Bo Lai, and Hong-Li Huang
Journal of Gastroenterology and Hepatology, 2010, Volume 25, Number 4, Page 772

Comments (0)

Please log in or register to comment.