Jump to ContentJump to Main Navigation

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R. / Tsongalis, Gregory J.

12 Issues per year

IMPACT FACTOR 2014: 2.707
Rank 6 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2014: 0.741
Source Normalized Impact per Paper (SNIP) 2014: 1.011
Impact per Publication (IPP) 2014: 2.310



Evaluation of a new automated electrochemiluminescent sex hormone-binding globulin (SHBG) immunoassay

Marijke Reynders1 / Ellen Anckaert2 / Johan Schiettecatte3 / Johan Smitz4





Corresponding author: Ellen Anckaert, RIA Laboratorium, Laarbeeklaan 101, 1090 Brussels, Belgium Phone: +32-2-4775057, Fax: +32-2-4775060,

Citation Information: Clinical Chemical Laboratory Medicine. Volume 43, Issue 1, Pages 86–89, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2005.013, January 2005

Publication History

April 22, 2004
October 7, 2004


Serum sex hormone-binding globulin (SHBG) regulates the cellular bioavailability of SHBG-bound steroid hormones. Since variations in SHBG levels may affect the concentration of free, i.e., biologically active testosterone in serum, SHBG levels are commonly measured as a supplement to total testosterone determination. The recently developed electrochemiluminescence Elecsys ® SHBG immunoassay was evaluated analytically on a Modular E170 (Roche Diagnostics, Mannheim, Germany) immunoanalyzer. Major differences in SHBG concentrations have been described among the commercially available methods; we therefore compared the new method with an established SHBG immunoradiometric assay (IRMA) in 99 routine serum samples. To provide reference values to clinicians, SHBG concentration was measured by Elecsys ® in 304 serum samples from healthy volunteers and several relevant clinical subgroups. The within-run and total imprecision coefficients of variation were ≤2.9% and ≤3.3%, respectively. Functional sensitivity was at least 0.74 nmol/L. Recoveries after dilution of high-concentration samples in low-titer human serum or in assay diluent were within the range of 85–110%. The Elecsys ® SHBG assay correlated well (r=0.98) with the SHBG immunoradiometric assay, but values were higher for the Elecsys ® assay (Passing Bablok regression analysis: slope 1.14, intercept +2.5). In healthy subjects and clinical subgroups, we confirmed the differences in SHBG values reported in the literature. The Elecsys ® SHBG immunoassay provides precision and reliability in combination with reduced turnaround time.

Keywords: immunoassay; sex hormone-binding globulin

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

A. Goto, Y. Song, B. H. Chen, J. E. Manson, J. E. Buring, and S. Liu
Diabetes, 2011, Volume 60, Number 1, Page 269
Johannes D. Veldhuis, Roy B. Dyer, Sergey A. Trushin, Olga P. Bondar, Ravinder J. Singh, and George G. Klee
Metabolism, 2014, Volume 63, Number 6, Page 783
T. Tao, E. P. Wickham, W. Fan, J. Yang, and W. Liu
European Journal of Endocrinology, 2010, Volume 163, Number 3, Page 399
T. Tao, S. Li, A. Zhao, Y. Zhang, and W. Liu
European Journal of Endocrinology, 2012, Volume 167, Number 5, Page 705
Xia Zhu, Yu Zhen, Tiezhu Mi, and Zhigang Yu
Marine Biotechnology, 2012, Volume 14, Number 4, Page 502
Eric L. Ding, Yiqing Song, JoAnn E. Manson, David J. Hunter, Cathy C. Lee, Nader Rifai, Julie E. Buring, J. Michael Gaziano, and Simin Liu
New England Journal of Medicine, 2009, Volume 361, Number 12, Page 1152
Patrick Vianna Garcia, Kélen Fabiola Arrotéia, Paulo Pinto Joazeiro, Suzana de Fátima Paccola Mesquita, Wilma de Grava Kempinas, and Luis Antonio Violin Pereira
Fertility and Sterility, 2011, Volume 96, Number 3, Page 739
D. Finas, M. Bals-Pratsch, J. Sandmann, R. Eichenauer, D. Jocham, K. Diedrich, P. Schmucker, and M. Huppe
Andrologia, 2006, Volume 38, Number 2, Page 48

Comments (0)

Please log in or register to comment.