Jump to ContentJump to Main Navigation

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R. / Tsongalis, Gregory J.

12 Issues per year

IMPACT FACTOR 2014: 2.707
Rank 6 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2014: 0.741
Source Normalized Impact per Paper (SNIP) 2014: 1.011
Impact per Publication (IPP) 2014: 2.310



Cervical human papillomavirus screening by PCR: advantages of targeting the E6/E7 region

Brian J. Morris1


Corresponding author: Brian J. Morris, Basic & Clinical Genomics Laboratory, School of Medical Sciences and Institute for Biomedical Research, Building F13, The University of Sydney, NSW 2006, Australia Phone: +61-2-9351 3688, Fax: +61-2-9351 2227,

Citation Information: Clinical Chemical Laboratory Medicine. Volume 43, Issue 11, Pages 1171–1177, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2005.203, October 2005

Publication History

June 29, 2005
September 5, 2005
Published Online:


PCR is a promising method for detection of human papillomavirus (HPV), the high-risk forms of which are responsible for cervical cancer. PCR primers that target the L1 or E1 region can be unreliable and may miss more advanced disease, whereas those directed at the E6 or E7 regions, which encode oncogenic products, are preferable because 1) the LI/E1 regions, but never the E6/E7 regions, are lost during integration of viral DNA into host genomic DNA, a process that can represent an integral component of progression from infection to tumorigenesis; and 2) the E6/E7 nucleotide sequence exhibits less nucleotide variation. The choice of region used for PCR has implications for HPV screening strategies in the clinical diagnosis and management of cervical cancer.

Keywords: cervical cancer; cervical dysplasia; cervical intrepithelial neoplasia (CIN); diagnostic testing; oncogenes; open reading frames E6, E7, E1, E2 and L1; papillomavirus, human; PCR; viral integration

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Patrick Roos, Paul A. Orlando, Richard M. Fagerstrom, and John W. Pepper
Scientific Reports, 2015, Volume 5, Page 8645
Daniel Weiss, Thomas Heinkele, and Claudia Rudack
Journal of Medical Virology, 2015, Volume 87, Number 5, Page 860
Ryan C. Chai, Duncan Lambie, Mukesh Verma, and Chamindie Punyadeera
Cancer Medicine, 2015, Volume 4, Number 4, Page 596
Linqian Wang, Baiping Wu, Junjun Li, and Liyu Chen
Journal of Medical Virology, 2015, Volume 87, Number 3, Page 516
Andreas Kaiser, Brigitte Jenewein, Haymo Pircher, Ursula Rostek, Pidder Jansen-Dürr, and Werner Zwerschke
Virus Genes, 2015, Volume 50, Number 1, Page 12
O.K. Vintermyr, O. Iversen, S. Thoresen, W. Quint, A. Molijn, S. de Souza, D. Rosillon, and K. Holl
Gynecologic Oncology, 2014, Volume 133, Number 2, Page 159
Tiatou Souho and Bahia Bennani
Journal of Virological Methods, 2014, Volume 196, Page 45
D. C. Damin, P. K. Ziegelmann, and A. P. Damin
Colorectal Disease, 2013, Volume 15, Number 8, Page e420
Benny Kirschner, Jette Junge, Katsiaryna Holl, Mats Rosenlund, Sabrina Collas de Souza, Wim Quint, Anco Molijn, David Jenkins, and Doris Schledermann
Acta Obstetricia et Gynecologica Scandinavica, 2013, Volume 92, Number 9, Page 1023
A. Trevisan, N. F. Schlecht, A. V. Ramanakumar, L. L. Villa, and E. L. Franco
Journal of General Virology, 2013, Volume 94, Number Pt_8, Page 1850
Wiebren A. Tjalma, Alison Fiander, Olaf Reich, Ned Powell, Andrzej M. Nowakowski, Benny Kirschner, Robert Koiss, John O'Leary, Elmar A. Joura, Mats Rosenlund, Brigitte Colau, Doris Schledermann, Kersti Kukk, Vasileia Damaskou, Maria Repanti, Radu Vladareanu, Larisa Kolomiets, Alevtina Savicheva, Elena Shipitsyna, Jaume Ordi, Anco Molijn, Wim Quint, Alice Raillard, Dominique Rosillon, Sabrina Collas De Souza, David Jenkins, and Katsiaryna Holl
International Journal of Cancer, 2013, Volume 132, Number 4, Page 854
Lina Michala, Elena Argyri, Elpida Tsimplaki, Artemis Tsitsika, Chryssa Bakoula, Aris Antsaklis, and Efstathia Panotopoulou
Gynecologic Oncology, 2012, Volume 126, Number 2, Page 207
Deepti Joshi and Gertrude Case Buehring
Breast Cancer Research and Treatment, 2012, Volume 135, Number 1, Page 1
Elisa Martró, María José Valencia, Antoni Tarrats, Eva Castellà, Mariona Llatjós, Sara Franquesa, Lurdes Matas, and Vicente Ausina
Enfermedades Infecciosas y Microbiología Clínica, 2012, Volume 30, Number 5, Page 225
A.P. Damin, D.C. Damin, and C.O.P. Alexandre
The Breast, 2007, Volume 16, Number 3, Page 222
I. Nazarenko, L. Kobayashi, J. Giles, C. Fishman, G. Chen, and A. Lorincz
Journal of Virological Methods, 2008, Volume 154, Number 1-2, Page 76
Christine F.W. Vermeulen, Ekaterina S. Jordanova, Károly Szuhai, Sandra Kolkman-Uljee, M. Albert Vrede, Alexander A.W. Peters, Ed Schuuring, and Gert Jan Fleuren
Cancer Genetics and Cytogenetics, 2007, Volume 175, Number 2, Page 132
Gaëlle Boulet, Caroline Horvath, Davy Vanden Broeck, Shaira Sahebali, and Johannes Bogers
The International Journal of Biochemistry & Cell Biology, 2007, Volume 39, Number 11, Page 2006
Brian J. Morris and Barbara R. Rose
Clinical Chemical Laboratory Medicine, 2007, Volume 45, Number 5
Anthony N. Snow and Jennifer Laudadio
Advances in Anatomic Pathology, 2010, Volume 17, Number 6, Page 394
Donna Dehn, Kathleen C. Torkko, and Kenneth R. Shroyer
Cancer, 2007, Volume 111, Number 1, Page 1
Gitana Maria Aceto, Angela Rosaria Solano, Maria Isabel Neuman, Serena Veschi, Annalisa Morgano, Sara Malatesta, Reinaldo Daniel Chacon, Carmen Pupareli, Mercedes Lombardi, Pasquale Battista, Antonio Marchetti, Renato Mariani-Costantini, and Ernesto Jorge Podestà
Breast Cancer Research and Treatment, 2010, Volume 122, Number 3, Page 671
C. E. Depuydt, G. A. V. Boulet, C. A. J. Horvath, I. H. Benoy, A. J. Vereecken, and J. J. Bogers
Journal of Cellular and Molecular Medicine, 2007, Volume 11, Number 4, Page 881
Christina S. Kong, Bonnie L. Balzer, Megan L. Troxell, Bruce K. Patterson, and Teri A. Longacre
The American Journal of Surgical Pathology, 2007, Volume 31, Number 1, Page 33

Comments (0)

Please log in or register to comment.