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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R. / Tsongalis, Gregory J.

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Automation of biochip array technology for quality results

Roisin M. Molloy1 / Robert I. Mc Connell2 / John V. Lamont3 / Stephen P. FitzGerald4

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Corresponding author: Mr. John Lamont, 55 Diamond Road, Crumlin, Co Antrim, BT29 4QY, UK Phone: +44 2894 422413, Fax: +44 2894 452912, All authors are employees of Randox Laboratories Ltd, Crumlin, UK, and were involved in the development of the Evidence Biochip Array analyser.

Citation Information: Clinical Chemical Laboratory Medicine. Volume 43, Issue 12, Pages 1303–1313, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2005.224, November 2005

Publication History

Received:
September 20, 2005
Accepted:
October 26, 2005
Published Online:
2005-11-28

Abstract

Background: Proteomics' requirement for simultaneous measurement of multiple markers is now possible with biochip array technology. Many laboratories utilise in-house, manual procedures for biochip fabrication and sample testing. Reproducibility and standardisation of biochip processes is vital to ensure quality of results and offer the best tool for elucidation of complex relationships between multiple proteins in diseased conditions.

Methods: Various novel control checks have been implemented in biochip fabrication, reagent manufacture, automation and imaging processes for the Evidence analyser. Reference spots enable location of discrete test regions on the surface of the biochip and simultaneous quantification of multiple markers. Performance and standardisation methods are presented.

Results: Formulation of dispense solution for discrete test regions had a direct effect on their shape, stability and integrity on the biochip surface. Assays for fertility hormones and drugs of abuse demonstrated excellent precision, stability and comparison with other commercial methods.

Conclusion: Control processes employed in the manufacture and analysis of Evidence components ensure reproducibility of assays for a range of routine and novel markers.

Keywords: automated biochip array; protein arrays; proteomics; quality control; standardisation

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