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Publication Date:
April 2005
ISSN:
1437-4331
DOI:
10.1515/CCLM.2005.027

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Clinical Chemistry and Laboratory Medicine (CCLM)

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Y-Chromosome short tandem repeat (STR) haplotypes in a Campania population sample

Giuseppe Calcagno1 / Giuseppe Labruna2 / Lucia Sacchetti3

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Corresponding author: Lucia Sacchetti, Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli “Federico II” and CEINGE Biotecnologie Avanzate, via S. Pansini 5, Naples, Italy Fax: +39-081-7462404,

Citation Information: Clinical Chemical Laboratory Medicine. Volume 43, Issue 2, Pages 163–166, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2005.027, April 2005

Publication History:
Received:
April 4, 2004
Accepted:
November 16, 2004

Abstract

Given the lack of information about Y haplotypes for Campania (southern Italy), we analyzed eight Y short tandem repeats in a sample of males from this region with the aim of establishing a Y-haplotype database that can be used for forensic purposes. The eight Y short tandem repeats were amplified by two PCR multiplex reactions: multiplex A for loci DYS19, DYS385, DYS392 and DYS393, and multiplex B for DYS389 I and II, DYS390, DYS391 and DYS385. The proportion of unique haplotypes was 100% (108 Y-chromosome haplotypes in 108 unrelated males) and the haplotype diversity discrimination index was 0.99. These data reflect the high heterogeneity of male lineage in our population and are similar to those obtained in other regions of southern Italy.

Keywords: capillary electrophoresis; haplotype; short tandem repeats; Y chromosome

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