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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Editorial Board Member: Gillery, Philippe / Kazmierczak, Steven / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Whitfield, John B.

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Automation and validation of a fast method for the assessment of in vivo oxidative stress levels

Cristina Vassalle1 / Claudio Boni2 / Pietro Di Cecco3 / Rudina Ndreu4 / Gian Carlo Zucchelli5

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Corresponding author: Dr. Cristina Vassalle, PhD, Istituto di Fisiologia Clinica-Area di ricerca CNR, Via Moruzzi 1, 56124, Pisa, Italy Phone: +39-050-3152199, Fax:+39-050-3152166

Citation Information: Clinical Chemical Laboratory Medicine. Volume 44, Issue 11, Pages 1372–1375, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2006.243, November 2006

Publication History

Received:
May 26, 2006
Accepted:
August 23, 2006
Published Online:
2006-11-07

Abstract

Background: The d-ROMs test for the evaluation of serum hydroperoxides (HP) is simple, reliable, and cheap. Furthermore, it can easily be adapted to automated analyzers. Changing from the manual to an automated procedure allows the simultaneous processing of a large number of samples in a greatly reduced time, avoiding manual handling of samples and reagents and reducing variability sources.

Methods: This study was performed to adjust the manual procedure to a routine automated method in the clinical laboratory. We carried out the d-ROMs test in sera from 90 subjects of both sexes (34 men and 56 women) with age ranging from 20 to 80 years (mean 51±14 years). All subjects were free from acute or chronic inflammatory disease, immunological disease and history or evidence of malignancy. Subjects were not on vitamin and/or antioxidant therapies.

Results: The detection limit of the assay was 40 AU. Linearity was observed up to 475 AU. The recovery ranged between 97% and 105%. Within- and between-run imprecision was <5%. The mean HP value was 304±8 AU, with no significant difference between men (291±10 AU) and women (311±11 AU). A significant positive correlation was observed between age and HP in the whole population (r=0.4, p=0.0002).

Conclusions: The automated test for the estimation of serum hydroperoxides represents a reliable and feasible procedure for increasing efficiency and reducing costs compared to the manual method, and is particularly suitable for evaluating oxidative stress in a variety of clinical conditions.

Clin Chem Lab Med 2006;44:1372–5.

Keywords: age; automated procedure; gender; manual method; oxidative stress

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