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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Editorial Board Member: Gillery, Philippe / Kazmierczak, Steven / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Whitfield, John B.

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IMPACT FACTOR 2013: 2.955
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Urinary cystatin C as a specific marker of tubular dysfunction

Marc Conti1 / Stéphane Moutereau2 / Mokhtar Zater3 / Karim Lallali4 / Antoine Durrbach5 / Philippe Manivet6 / Pascal Eschwège7 / Sylvain Loric8

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Corresponding author: Prof. Sylvain Loric, Laboratoire de Biochimie et Génétique, CHU Henri Mondor de Créteil, 51, avenue du Maréchal de Lattre de Tassigny, 94010 Créteil cedex, France Phone: +33-1-49812847, Fax: +33-1-49812842,

Citation Information: Clinical Chemical Laboratory Medicine. Volume 44, Issue 3, Pages 288–291, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2006.050, March 2006

Publication History

Received:
October 11, 2005
Accepted:
December 16, 2005

Abstract

Background: Cystatin C (CST3), a strong inhibitor of cysteine proteinases, is freely filtered by the kidney glomerulus and is reabsorbed by the tubules, where it is almost totally catabolized, with the remainder then eliminated in urine. In tubular diseases, it seems sensible to postulate that CST3 degradation would be reduced and consequently an increase in its urinary elimination would be observed.

Methods: We report here the development of an automatic quantitative assay to measure CST3 concentrations in urine using a Behring N-Latex Cystatin C kit on a BNII laser nephelometer. We tested its clinical relevance on several kidney disease patients.

Results: This assay is sensitive (limit of detection 0.008 mg/L) and precise (within- and between-day CVs <4%). Reference values for freshly collected urine samples range from 0.03 to 0.18 mg/L. Mean urine CST3 concentrations obtained from 52 patients with kidney tubular disease (4.31±3.85 mg/L) were significantly higher than those for 60 controls (0.096±0.044 mg/L; p<0.0001) and 47 glomerular disease patients (0.106±0.133 mg/L; p<0.0001).

Conclusion: Increased urinary CST3 concentrations allow the accurate detection of tubular dysfunction among pure and mixed nephropathies. Because of its ability to be processed on automated clinical chemistry analyzers, this assay could easily be used as an adjunct to the standard panel used to screen kidney pathologies, even in emergency situations.

Keywords: cystatin C; kidney; tubular marker; tubulopathy; urine

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