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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Editorial Board Member: Gillery, Philippe / Kazmierczak, Steven / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Whitfield, John B.

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Autoantibody profiling of patients with autoimmune thyroid disease using a new multiplexed immunoassay method

Renato Tozzoli1 / Danilo Villalta2 / Graziano Kodermaz3 / Marcello Bagnasco4 / Elio Tonutti5 / Nicola Bizzaro6

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Corresponding author: Nicola Bizzaro, MD, Laboratory of Clinical Pathology, Ospedale Civile, 33028 Tolmezzo (UD), Italy Phone: +39-0433-488261

Citation Information: Clinical Chemical Laboratory Medicine. Volume 44, Issue 7, Pages 837–842, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2006.137, June 2006

Publication History

Received:
December 5, 2005
Accepted:
March 10, 2006
Published Online:
2006-06-16

Abstract

Background: It has been proposed that only anti-thyroid-peroxidase (TPOAb) and not anti-thyroglobulin (TgAb) antibodies should be assayed in the diagnosis of autoimmune thyroid disease (AITD).

Methods: We analyzed the thyroid autoantibody profile with a multiplex bead array system, which allows simultaneous measurement of thyroid autoantibodies (FIDIS, BioMedical Diagnostics, Marne La Vallée, France). A total of 151 AITD patients (111 Hashimoto's thyroiditis and 40 Graves' disease) and 414 control patients were recruited to the study.

Results: The diagnostic sensitivity of TPOAb was greater than that of TgAb in both Hashimoto's thyroiditis (91.9% vs. 82.5%) and Graves' disease (82.5% and 52.5%, respectively), whereas the diagnostic specificity was similar (92.7% for TPOAb and 92.4% for TgAb).

Conclusions: The presence of isolated positivity for TgAb in 6% of the Hashimoto's thyroiditis patients indicates that the use of only the TPOAb test does not guarantee sufficient diagnostic sensitivity. In the light of recent findings relating to the pathogenetic role of TgAb and TPOAb in Hashimoto's thyroiditis, knowledge of the autoantibody profile could allow the identification of sub-groups of patients with a different degree of activation of the thyroid autoimmune process.

Clin Chem Lab Med 2006;44:837–42.

Keywords: Graves' disease; Hashimoto's thyroiditis; multiplex bead array; thyroglobulin antibodies; thyroperoxidase antibodies

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