Jump to ContentJump to Main Navigation
Show Summary Details

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.


IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238

249,00 € / $374.00 / £187.00*

Online
ISSN
1437-4331
See all formats and pricing

 


Select Volume and Issue
Loading journal volume and issue information...

Fifteen polymorphisms in endothelin-1, endothelin-2 and endothelin-receptor-A genotyped by four duplex assays and seven simple assays on a LightCycler using hybridization probes

Farhad Arjomand-Nahad1 / Olfert Landt2 / Karl Stangl3 / Konstanze Diefenbach4 / Ivar Roots5

1.

2.

3.

4.

5.

Corresponding author: Farhad Arjomand-Nahad, Institute of Clinical Pharmacology, Campus Charité Mitte, Charité University Medical Center, Schumannstr. 20/21, 10117 Berlin, Germany Phone: +49-30-450525275, Fax: +49-30-450525926,

Citation Information: Clinical Chemical Laboratory Medicine. Volume 44, Issue 8, Pages 929–932, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2006.180, August 2006

Publication History

Received:
March 20, 2006
Accepted:
May 8, 2006

Abstract

Endothelins (EDNs) are peptides, produced by various tissues, with potent vasoactive and mitogenic properties. Endothelin actions are mediated via specific G protein-coupled receptors of two subtypes, endothelin-receptor-A (EDNRA) and endothelin-receptor-B (EDNRB). Some polymorphisms of the EDN1, EDN2 and EDNRA genes may influence susceptibility to vascular diseases. Thus, genotyping for polymorphisms of these genes may represent a tool for predicting individual susceptibility to vascular diseases. Here, we report 11 fluorescence resonance energy transfer (FRET) assays for the detection of 15 polymorphisms, because the assays used in previous studies (allele-specific PCR and restriction fragment-length polymorphism assays) for some of these polymorphisms are laborious and time-consuming. The newly developed assays are fast and work without expensive ready-to-use mixtures.

Clin Chem Lab Med 2006;44:929–32.

Keywords: duplex assay; EDN1; EDN2; endothelin-receptor-A (EDNRA); genotyping; LightCycler; single nucleotide polymorphism (SNP)

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Konstanze Diefenbach, Farhad Arjomand-Nahad, Christian Meisel, Ingo Fietze, Karl Stangl, Ivar Roots, and Karla Köpke
Genetic Testing, 2006, Volume 10, Number 3, Page 163

Comments (0)

Please log in or register to comment.