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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Editorial Board Member: Gillery, Philippe / Kazmierczak, Steven / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Whitfield, John B.

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Distinct alleles of mannose-binding lectin (MBL) and surfactant proteins A (SP-A) in patients with chronic cavitary pulmonary aspergillosis and allergic bronchopulmonary aspergillosis

Mudit Vaid1 / Savneet Kaur2 / Helen Sambatakou3 / Taruna Madan4 / David W. Denning5 / P. Usha Sarma6

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Corresponding author: Professor David W. Denning, Education and Research Centre, Wythenshawe Hospital, Southmoor Rd, Manchester M23 9LT, UK Phone: +44-161-2915811, Fax: +44-161-2915806,

Citation Information: Clinical Chemical Laboratory Medicine. Volume 45, Issue 2, Pages 183–186, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2007.033, February 2007

Publication History:
Received:
September 3, 2006
Accepted:
November 10, 2006
Published Online:
2007-02-20

Abstract

Background: Distinct host immune status predisposes to different forms of pulmonary aspergillosis.

Methods: Patients with chronic cavitary pulmonary aspergillosis (CCPA; n=15) or allergic bronchopulmonary aspergillosis (ABPA; n=7) of Caucasian origin were screened for single nucleotide polymorphisms (SNPs) in the collagen region of surfactant proteins A1 (SP-A1) and A2 (SP-A2) and mannose binding lectin (MBL).

Results: The T allele at T1492C and G allele at G1649C of SP-A2 were observed at slightly higher frequencies in ABPA patients (86% and 93%) than in controls (63% and 83%), and the C alleles at position 1492 and 1649 were found in higher frequencies in CCPA patients (33% and 25%) than in ABPA patients (14% and 7%) (all p>0.05). However, the CC genotype at position 1649 of SP-A2 was significantly associated with CCPA (χ2=7.94; pcorr≤0.05). Similarly, ABPA patients showed a higher frequency of the TT genotype (71%) at 1492 of SP-A2 than controls (43%) and CCPA patients (41%) (p>0.05). In the case of MBL, the T allele (OR=3.1, range 1.2–8.9; p≤0.02) and CT genotype (χ2=6.54; pcorr≤0.05) at position 868 (codon 52) were significantly associated with CCPA, but not with ABPA. Further analysis of genotype combinations at position 1649 of SP-A2 and at 868 of MBL between patient groups showed that both CC/CC and CC/CT SP-A2/MBL were found only in CCPA patients, while GG/CT SP-A2/MBL was significantly higher in CCPA patients in comparison to ABPA patients (p≤0.05). SNPs analysed in SP-A1 did not differ between cases and controls.

Conclusions: Distinct alleles, genotypes and genotype combinations of SP-A2 and MBL may contribute to differential susceptibility of the host to CCPA or ABPA.

Clin Chem Lab Med 2007;45:183–6.

Keywords: allergic bronchopulmonary aspergillosis; Aspergillus fumigatus; chronic cavitary pulmonary aspergillosis; mannose-binding lectin; surfactant protein A

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